TABLE 4

hflB and hflB-proximal suppressors

Strain no. NCMSuppressorBackgroundTotal occurrencesDoubling timea(min)Behavior on Luria agar platesb
4284F plasmid in rrmJDouble1120cTinyRT
4266-4270IS186 in rrmJDouble5120cTinyRT
4272M19KDouble111037
4366S140RL394A1110dcsRT
4276A142EDouble3110Small37
4374L394A110csRT
4417fs110csRT
4286A163VDouble2150dSmallRT
4369L394A110cColorRT
4378A205TL394A1110csRT
4439A207Yfs1110cRT
4279F224LeDouble1110Small37 → suppressors
4388C249YL394A2110cs37
4408fs110cs37
4356D257EL394A1110dSmall + cs37
4446G269Cfs1110dRT
4265E273DDouble1110dSmallRT
4368R274LL394A1110RT
4420Δ289-291fs2110dTransparentRT
4421110d
4380A299VL394A1110dTransparent37
4389R302CL394A290dcs37
439390dcs37
4424A309Sfs1110dOdd color37
4373G314SL394A1160cRT
4407R318Cfs1110Small + cs37
4371Q319stopL394A1120dSmallRT
4461S360YLoop 51100cd37
4444S360Ffs1110dTransparent RT
4387G361SL394A1110cs37
4360L367QL394A1150cOdd colorRT
4422A391Efs1110RT
4279E408KeDouble1110Small37 → suppressors
4364F451CL394A1180dOdd colorRT
4273G491DDouble2110dSmall37
4274110dSmall37
4465D542YLoop 71100d37
4411stop649S + extensionfs1120cTiny37
  • “fs” refers to the frameshifted protein.

  • a Minimal medium, pH 5.5, containing 0.5 mm NH4+ and 0.1% glucose. The doubling time of a wild-type strain was ∼50 min, and the doubling time of ΔC-terminal varied from 100 to 120 min in different experiments.

  • b Appearance on initial purification plates. cs, cold sensitive; growth at 37° but not at room temperature (RT). Superscript gives temperature at which suppressor was isolated.

  • c Doubling time increased as ammonium was depleted. This phenotype was common among strains isolated at RT.

  • d Lag before constant doubling time was reached.

  • e These two lesions were found in the same suppressor strain.