TABLE 2

Primers used in this study for BAC and genomic screening

PrimerSequenceFunction
Hit ScreenF5′-AAGCTTACTGTCAGCTCGAG-3′For screening Hit clones
S73FixScr5′-GGGAGCAGCGCACCCAACGCCCCTATGGCT-3′For screening 73 fixed clones
S409FixScr5′-ACAAGCAGCCGGAGGAGCGCTATGAGCGCA-3′For screening 409 fixed clones
Ex2/F5′-TGTTGGTCAACTGGTCAGTCT-3′For sequencing exon 2
Ex2/R5′-AGGACAGAGGTTGGTGACAAT-3′For sequencing exon 2 and for screening hit-and-fix clones
RecEx9Scr5A5′-CCTGATCTGGTGAATCGGATCATCAAGC-3′For sequencing part of exon 9
RecEx9Scr3A5′-CAGATACTGCACGCTGAAGGTACAATAC-3′For sequencing part of exon 9 and for screening hit-and-fix clones
vga9-for5′-CTGCAGCACGGACAATAAACCTCC-3Mitf: specific for vga9 mutation
mitf-rev5′-AAACAAGGCATCCCGAGGCACC-3′Mitf: for screening vga9 mutation
Mitf-2BtestF5′-CAGGGACGTTAAACAGGATAGT-3′For screening deletions
Mitf-2BtestR5′ -AGGTCCTGAAGTCATCAGGA-3′For screening deletions
CMr-F5′-AGCATTCTGCCGACATGGAAGC-3′BAC clone forward
CMr-R5′-CACGACGATTTCCGGCAGTTTC-3′BAC clone reverse