TABLE 1

Primers used in the study for BAC recombineering

PrimerSequence
Ser73
Ser73HitF5′-ACATGCCAGCCAAGTCCTGAGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGAAGCTTACTGTCAGCTCGAG-3′
Ser73HitB5′-AGTTAATGGAATGTAAGAAACAGAGGAGAAGAGGAGACGTGAATTACCTCTTTTTCACAGTTGGAGTTAAGAGTGAGCATCTCGAGCTGACAGTAAGCTT-3′
Ser73FixF5′-ACATGCCAGCCAAGTCCTGAGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCACCCAACGCCCCTATGGCT-3′
Ser73FixB5′-AGTTAATGGAATGTAAGAAACAGAGGAGAAGAGGAGACGTGAATTACCTCTTTTTCACAGTTGGAGTTAAGAGTGAGCATAGCCATAGGGGCGTTGGGTG-3′
Ser409
Ser409HitF5′-GGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTCGCCAGGAGCTTCAAAAACAAGCAGCCAAGCTTACTGTCAGCTCGAG-3′
Ser409HitB5′-AGTCTCCTGAAGAAGAGAGGGAGCGGTCCGTGCAGAGGCAGAGCAAGGCAGGCTCGCTAACACGCATGCTCCGTTTCTTCCTCGAGCTGACAGTAAGCTT-3′
Ser409FixF5′-GGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTCGCCAGGAGCTTCAAAAACAAGCAGCCGGAGGAGCGCTATGAGCGCA-3′
Ser409FixB5′-AGTCTCCTGAAGAAGAGAGGGAGCGGTCCGTGCAGAGGCAGAGCAAGGCAGGCTCGCTAACACGCATGCTCCGTTTCTTCTGCGCTCATAGCGCTCCTCC-3′
Del(int1/2A)
Mitf galK FCTGCCTGAAACCTTGCTATGCTGGAAATGCTAGAATACAGTCACTACCAGCCTGTTGACAATTAATCATCGGCA
Mitf galK RACTTGGCTGGCATGTTTATTTGCTAAAGTGGTAGAAAGGTACTGCTTTACTCAGCACTGTCCTGCTCCTT
galK del(int1/2a)upCTGCCTGAAACCTTGCTATGCTGGAAATGCTAGAATACAGTCACTACCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCAAGT
galK del(int1/2a)lowACTTGGCTGGCATGTTTATTTGCTAAAGTGGTAGAAAGGTACTGCTTTACCTGGTAGTGACTGTATTCTAGCATTTCCAGCATAGCAAGGTTTCAGGCAG
Del(2B/int2)
Mitf2B galK FACCTGGAAAACCCCACCAAGTACCACATACAGCAAGCTCAGAGGCACCAGCCTGTTGACAATTAATCATCGGCA
Mitf2B galK RCCTGGGCACTCACTCTCTGCCCTGCTCTGCTCCTCAAACTTATAAAATGCTCAGCACTGTCCTGCTCCTT
Mitf2B SACCTGGAAAACCCCACCAAGTACCACATACAGCAAGCTCAGAGGCACCAGGCATTTTATAAGTTTGAGGAGCAGAGCAGGGCAGAGAGTGAGTGCCCAGG
Mitf2B ASCCTGGGCACTCACTCTCTGCCCTGCTCTGCTCCTCAAACTTATAAAATGCCTGGTGCCTCTGAGCTTGCTGTATGTGGTACTTGGTGGGGTTTTCCAGGT
Del(int2)
Mitf intr2 galK FCCAACAGCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGCCTGTTGACAATTAATCATCGGCA
Mitf2B galK RCCTGGGCACTCACTCTCTGCCCTGCTCTGCTCCTCAAACTTATAAAATGCTCAGCACTGTCCTGCTCCTT
Mitf intr2 SCCAACAGCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGGAGCAGAGCAGG
Mitf intr2 ASATGCACGACGCTCGAGAGTGCGTGTTCATACCTGGGCACTCACTCTCTGCCCTGCTCTGCTCCTCAAACTTATAAAATGC