TABLE 1

Yeast strains used in this study

StrainBackgroundRelevant genotypeReferences or source
Σ1278bΣ1278bMATα SAL1 MKT1-30G CAT5-91M MIP1-661TF. Hilger (Gembloux)
EM93aProgenitor of S288CMATa/α SAL1/sal1-1 MKT1-30G/MKT1-30G CAT5-91M/CAT5-91I MIP1-661T/MIP1-661AMortimer and Johnston (1986)
UCC1158RM11-1aMATa ho∷loxP-KAN-loxP leu2Δ0 ura3Δ0 SAL1 MKT1-30G CAT5-91M MIP1-661TMortimer et al. (1994)
BY4716S288CMATα lys2Δ0 sal1-1 MKT1-30D CAT5-91I MIP1-661ABrachmann et al. (1998)
S288CS288CMATα mal gal2 sal1-1 MKT1-30D CAT5-91I MIP1-661AAdams et al. (1997); Mortimer and Johnston (1986)
UCC1166SK1MATα ho∷LYS2 leu2Δ(Asp18-EcoRI) lys2Δ0 ura3Δ0 SAL1 MKT1-30G CAT5-91M MIP1-661TBarbara Garvik (Hartwell Lab)
YDS3W303MATα ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3 RAP1 wt SAL1 MKT1-30G CAT5-91M MIP1-661ALori Sussel (Shore Lab)
UCC8407YPS163MATa ho∷loxP-KANMX4-loxP SAL1 MKT1-30G CAT5-91M MIP1-661TThis study
UCC8409YPS1000MATa ho∷loxP-KANMX4-loxP SAL1 MKT1-30G CAT5-91M MIP1-661TThis study
UCC8411YPS1009MATa ho∷loxP-KANMX4-loxP SAL1 MKT1-30G CAT5-91M MIP1-661TThis study
UCC8062-1Diploid RM11MATa/α LEU2/leu2Δ0 LYS2/lys2Δ0 ura3Δ0/ura3Δ0 ho∷loxP-KAN-loxP/ho∷loxP-KAN-loxPThis study
UCC8088-1Diploidized BY4716MATa/α lys2Δ0/lys2Δ0This study
UCC8240S288CMATa MKT1-30D ura3Δ0This study
UCC8241S288CMATa MKT1-30G ura3Δ0This study
UCC8334S288CMATα lys2Δ0 [S288C rho+]This study
UCC8335S288CMATα lys2Δ0 [S288C rho+]This study
UCC8338S288CMATα lys2Δ0 [RM11 rho+]This study
UCC8339S288CMATα lys2Δ0 [RM11 rho+]This study
UCC8340RM11MATα ho∷loxP-KAN-loxP lys2Δ0 ura3Δ0 [S288C rho+]This study
UCC8341RM11MATα ho∷loxP-KAN-loxP lys2Δ0 ura3Δ0 [S288C rho+]This study
UCC8344RM11MATα ho∷loxP-KAN-loxP lys2Δ0 ura3Δ0 [RM11 rho+]This study
UCC8356S288CMATa MKT1-30G sal1-1 CAT5-91I MIP1-661A ura3Δ0This study
UCC8357S288CMATa MKT1-30G sal1-1 CAT5-91I MIP1-661T ura3Δ0This study
UCC8358S288CMATα MKT1-30G sal1-1 CAT5-91M MIP1-661A ura3Δ0This study
UCC8359S288CMATα MKT1-30G sal1-1 CAT5-91M MIP1-661T ura3Δ0This study
UCC8360S288CMATa MKT1-30G SAL1 CAT5-91I MIP1-661A ura3Δ0This study
UCC8361S288CMATα MKT1-30G SAL1 CAT5-91I MIP1-661T ura3Δ0This study
UCC8362S288CMATa MKT1-30G SAL1 CAT5-91M MIP1-661A ura3Δ0This study
UCC8363S288CMATα MKT1-30G SAL1 CAT5-91M MIP1-661T ura3Δ0This study
UCC8372S288CMATα MKT1-30G SAL1 CAT5-91M MIP1-661T ura3Δ0 [RM11 rho+]This study
UCC8374S288CMATα MKT1-30G SAL1 CAT5-91M MIP1-661T ura3Δ0 [RM11 rho+]This study
UCC8376S288CMATα MKT1-30G SAL1 CAT5-91M MIP1-661T ura3Δ0 [S288C rho+]This study
UCC8378S288CMATα MKT1-30G SAL1 CAT5-91M MIP1-661T ura3Δ0 [S288C rho+]This study
UCC8413Hybrid BY4716 × RM11MATa/α LEU2/leu2Δ0 LYS2/lys2Δ0 URA3/ura3Δ0 ho/ho∷loxP-kan-loxPThis study
  • a A kind gift of Joshua Akey's lab.