TABLE 4

Analysis of mitochondrial introns

PCR confirmation
IntronGeneBYRM
aI1COX1+
aI5aCOX1+
aI5bCOX1+
aI5cCOX1+
r121S_rRNA+
  • Total DNA (nuclear and mitochondrial) from either the BY4716 or the RM11-1a strain was used as template in PCR reactions. Primer pairs (see Table S1) annealing to the exons that flank the listed introns were used. A minus (−) indicates that the PCR reaction amplified the shorter product, consistent with the absence of the respective intron in RM. A plus (+) indicates that the presence of the respective intron in BY was confirmed by PCR (see Table S1 for primers). Intron names are according to Pel and Grivell (1993).