TABLE 1

Strains and plasmids used in this study

Strain/plasmidGenotypeReference
CAB9MATa his3ΔHindIII leu2-3,112 lys2 ura3-52This study
CAB81MATα ade2 arg8Δ∷hisG leu2-3,112 lys2 ura3-52 cox18Δ∷KanMX4 yme1Δ∷URA3This study
CAB115MATα ade2 arg8Δ∷hisG leu2-3,112 lys2 ura3 cox18Δ∷KanMX4 [YEpNB6]This study
CAB115/1-2MATα ade2 arg8Δ∷hisG leu2-3,112 lys2 ura3 cox18Δ∷KanMX4 mgr1-1 [YEpNB6]This study
CAB115/2-8MATα ade2 arg8Δ∷hisG leu2-3,112 lys2 ura3 cox18Δ∷KanMX4 mgr3-2 [YEpNB6]This study
CUY564MATα ade2-101 ade3-24 leu2-3,112 ura3-52T. Huffaker
DFS188MATa arg8Δ∷hisG leu2-3,112 lys2 ura3-52Saracco and Fox (2002)
JKR101MATα ade2 his4-519 leu2-3,112 lys2 ura3-ΔThis study
HLF636MATa arg8Δ∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 cox18Δ∷URA3 mgr3Δ∷KanMX4 [pNB189]This study
HLF649MATa arg8Δ∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 cox18Δ∷URA3 mgr3Δ∷KanMX4This study
HLF691MATα ade2 leu2-3,112 ura3 trp1-1 cox18Δ∷KanMX4 mgr3Δ∷kanMX4 yme1Δ∷URA3This study
HFC1MATa arg8Δ∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 cox18Δ∷URA3 mgr1Δ∷KanMX4 [pNB189]This study
NB40-3CMATa arg8∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 [rho+ cox2-62]Bonnefoy and Fox (2000)
SCS59MATa arg8Δ∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 cox18Δ∷URA3Saracco and Fox (2002)
SCS207MATa arg8Δ∷hisG his3ΔHinDIII leu2-3,112 lys2 ura3-52 cox18Δ∷URA3 mgr3-1 [pNB189]This study
pNB189LEU2 OXA1Gift from N. Bonnefoy
YEpNB6URA3 OXA1Bonnefoy et al. (1994a)
pRS314-YME1TRP1 CEN6/ARSH4 YME1Weber et al. (1996)
pRS314A6TRP1 CEN6/ARSH4 yme1(K327R)Weber et al. (1996)
pRS314Z3TRP1 CEN6/ARSH4 yme1(E541A)Weber et al. (1996)
  • All strains are congenic to D273-10B except CUY564, which is congenic to S288C. All strains carry wild-type rho+ mtDNA except NB40-3C, whose rho+ mtDNA carries the cox2-62 deletion.