TABLE 4

Stepwise efficiency of creating deletions using the P{wHy} mobile element

Set of deletions
01C0301D0101D0901F0402H09
Steps of the procedurewy+w+ywy+w+ywy+w+ywy+w+ywy+w+yTotal
Total crosses387387187018701539153910151015344234428253
hobo mobilization37 (10)19 (5)285 (15)363 (19)404 (26)168 (11)58 (6)22 (2)96 (3)19 (1)1471
Survival and fertile34 (92)17 (89)272 (95)351 (97)354 (88)152 (90)40 (69)17 (77)96 (100)19 (100)1352
Genomic events16 (47)11 (65)65 (24)215 (61)233 (66)37 (24)18 (45)9 (53)50 (52)10 (53)664
Mapped derivatives13 (81)7 (64)53 (82)164 (76)153 (66)ND13 (72)ND34 (68)8 (80)445 (72)
Candidate deletions9 (69)4 (57)47 (89)119 (73)127 (83)ND9 (69)ND31 (91)4 (50)350 (78)
Nonredundant deletions9 (100)4 (100)35 (74)105 (88)114 (90)ND8 (89)ND26 (84)4 (100)305 (87)
Validated deletionsND4 (100)ND100 (95)113 (99)NDndNDNDND217 (95)
Deletions <60 kb9 (100)4 (100)27 (77)74 (74)78 (69)ND8 (100)ND25 (96)2 (50)228
  • Total crosses indicates the number of crosses set up for each set. All other entries represent the number of the chromosomes belonging to the indicated step. The w+y class includes a minority that is phenotypically wy, but structurally confirmed as w+y; deletions are validated by genetic complementation test. The percentage of the deletions relative to the number of deletions of the previous step is in parentheses, when known; ND, no data. Total is the overall number of chromosomes for each step. 01D01 w+y and 01 D09 wy+ deletion sets have been presented in Huet et al. (2002) and are shown here for comparison.