TABLE 2

Effects of repair mutations on ZFN-induced targeting

GenotypeDonoraParentsb% yielderscProgenyd% ryery/parentfHR/tot (% HR)g
A. In the male germline
1wtL95885,76111.06.637/209 (17.7)
2spnA057/+L67874,04210.26.212/110 10.9)
3spnA093A/+L131827,8569.25.525/174 (14.4)
4spnA057/093AL123869,26415.011.32/109 (1.8)
5spnA093A/093AL44733,80012.210.53/86 (3.5)
6spnA057/093A mei-W681/k05603L133688,40913.08.211/178 (6.2)
7wtC35912,19114.08.711/88 (12.5)
8spnA093A/+C139788,7497.84.95/149 (3.4)
9spnA093A/+ mei-W681/+C45842,9989.66.42/44 (4.5)
10spnA093A/093AC8269.55,6848.45.80/132 (0)
11spnA093A/093A mei-W681/1C67854,08811.57.00/131 (0)
12wtL49883,3017.34.923/145 (15.9)
13okrAA/+L60774,7746.65.329/95 (30.5)
14okrAA/AGL78805,7719.67.17/95 (7.4)
1wtL95885,76111.06.637/209 (17.7)
15lig4169L134638,7185.83.8123/268 (45.9)
16lig4169 spnA057/093AL30601,59513.77.39/110 (8.2)
4–6hspnA−/−L3007621,47313.79.816/373 (4.3)
7wtC35912,19114.08.711/88 (12.5)
17lig4169C93605,7474.52.822/80 (27.5)
18lig4169 spnA057/093AC61594,55411.08.20/263 (0)
10, 11ispnA−/−C14976.59,7729.76.40/263 (0)
B. In the female germline
1wtL11084.511,85313.114.170/269 (26.0)
2spnA057/+L8087.57,59712.712.016/87 (18.4)
3spnA093A/+L1609317,02316.217.256/214 (26.2)
6spnA057/093A mei-W681/k05603L59411,11510.72.01/80 (1.3)
7wtC43883,61112.410.418/89 (20.2)
8spnA093A/+C1559613,16516.013.616/161 (9.9)
9spnA093A/+ mei-W681/+C45894,38511.211.04/38 (9.5)
11spnA093A/093A mei-W681/1C395188212.12.70/101 (0)
12wtL74827,36012.112.065/165 (39.4)
13okrAA/+L92847,40013.210.627/86 (31.4)
1wtL11084.511,85313.114.170/269 (26.0)
19lig4169/+L95879,30816.215.932/81 (39.5)
20lig4169/169L62686,0727.06.876/87 (87.4)
7wtC43883,61112.410.418/89 (20.2)
21lig4169/169C77656,2056.65.445/90 (50.0)
  • a The donor DNA was provided in linear (L) or circular (C) form.

  • b Number of heat-shocked parents whose progeny were scored.

  • c Percentage of those parents that gave at least one ry offspring.

  • d Total number of offspring scored.

  • e Percentage of all offspring that were ry mutants.

  • f Average number of ry mutants per parent.

  • g Number of HR products over total analyzed, and HR as a percentage of total.

  • h Collected data for all spnA−/− combinations with linear donor: genotypes 4, 5, 6. For comparison with lig4 spnA−/−.

  • i Collected data for all spnA−/− combinations with circular donor: genotypes 10 and 11. For comparison with lig4 spnA−/−.