TABLE 2

Annotation of verified nucleotide changes

TotalNoncodingSynonymousNonsynonymousNonsense
All103821092
Standard7558692
Anomalous2824400
  • Lesions classified as “standard” are more likely to have arisen by EMS mutagenesis and have functional consequence. Lesions classified as “anomalous” are more likely to have arisen by gene conversion and to not have functional consequence. Five standard lesions (three noncoding, one synonymous, and one nonsynonymous mutation in the Cad99C gene) were located in the gene conversion clusters (see Figure 2, B and C) and have been shown to be gene conversion events.