TABLE 2

Comparison of expected cdc13-1 suppressors with actual results and testing of novel suppressors in an alternative genetic background

Gene deletionSuppressor of cdc13-1 in BY4741 (this work)Suppressor of cdc13-1 in W303 (this work)Previously documented suppressor of cdc13-1
rad9Δ++NA+
chk1Δ++NA+
ddc1Δ++NA+
rad24Δ++NA+
rad17Δ++NA+
upf3Δ++NA+
nmd2Δ++NA+
nam7Δ++NA+
exo1Δ++NA+
bub2ΔNA+
mec3ΔaNA+
bmh1Δ+NA+ (Downey et al. 2006)
san1Δ++NA+ (Downey et al. 2006)
pif1Δ+bNA+ (Downey et al. 2006)
cgi121Δ+cNA+ (Downey et al. 2006)
ebs1Δ+NA+ (Downey et al. 2006)
scs2Δ+++
poc4Δ++
snc2Δ+++
pkh3Δ++
pda1Δ++
oca1Δ++
oca2Δ++
siw14Δ (oca3Δ)++
oca4Δ++
oca6Δ++
oca5Δ+
  • ++, yes, a strong suppressor; +, yes; −, no.

  • a Deletion of MEC3 rescues cdc13-1 but was not tested in robotic assays due to slow growth.

  • b Deletion of PIF1 rescues cdc13-1 (Downey et al. 2006) but was not tested further in robotic assays due to slow growth.

  • c Deletion of CGI121 rescues cdc13-1 (Downey et al. 2006) but was not detected in robotic assays subsequent to initial scoring.