TABLE 1

Genes whose deletion rescues temperature sensitivity of cdc13-1

Strong suppressorsSuppressors
ORFGeneORFGeneORFGeneORFGeneORFGeneORFGene
YOL138CRTC1aYBR147WRTC2YKL087CCYT2YKL008CLAC1YAL023CPMT2YNL224CSQS1
YER177WBMH1YHR087WRTC3YGL078CDBP3YJL134WLCB3YPL144WPOC4YLR119WSRN2
[YFL023WBUD27]YNL254CRTC4YFL001WDEG1YNL323WLEM3YIL160CPOT1YHR066WSSF1
YBR274WCHK1YOR118WRTC5YKR035W-ADID2YLR451WLEU3YBL068WPRS4YBR283CSSH1
[YJR109CCPA2]YPL183W-ARTC6YGR227WDIE2YDL051WLHP1[YDL006WPTC1]YLR150WSTM1
YIL036WCST6YDL119CYDL119CYKL213CDOA1YJR070CLIA1YDR496CPUF6YDR320CSWA2
YPL194WDDC1YGR201CYGR201CYDR206WEBS1YNL307CMCK1YPR191WQCR2YNL081CSWS2
[YNL080CEOS1]YIL055CYIL055CYBL047CEDE1YPL098CMGR2YKR055WRHO4YLR354CTAL1
YOR033CEXO1YIL057CYIL057CYKL048CELM1YLR035CMLH2YNL180CRHO5YIL011WTIR3
YCR034WFEN1YIL161WYIL161WYDR512CEMI1YIL110WMNI1YIL053WRHR2YPR074CTKL1
YMR058WFET3YLR404WYLR404WYMR015CERG5YPR045CMNI2YLR453CRIF2YER007C-ATMA20
[YLR192CHCR1]YIL079CAIR1[YML008CERG6]YIR002CMPH1YIL066CRNR3YJR014WTMA22
YOL095CHMI1YER073WALD5YER145CFTR1YKL062WMSN4YDL082WRPL13AYGR260WTNA1
YMR080CNAM7YPL061WALD6YPL262WFUM1YPR134WMSS18[YKL006WRPL14A]YNL070WTOM7
YHR077CNMD2YGL148WARO2YBL016WFUS3YBR057CMUM2YBR084C-ARPL19AYNL121CTOM70
YOR209CNPT1YDR101CARX1YIL097WFYV10YHR004CNEM1YPL079WRPL21BYIL138CTPM2
YPL052WOAZ1[YMR116CASC1]YOR183WFYV12YLR363CNMD4YFR031C-ARPL2AYPL030WTRM44
YDL232WOST4YKL185WASH1YMR307WGAS1YER002WNOP16YLR406CRPL31BYLR425WTUS1
YOR368WRAD17YBL089WAVT5YJR040WGEF1YNL183CNPR1YOR234CRPL33BYER151CUBP3
YER173WRAD24YIL124WAYR1YGL020CGET1YNL099COCA1YER056C-ARPL34AYFR010WUBP6
YDR217CRAD9YKR099WBAS1YHL031CGOS1YNL056WOCA2YDL191WRPL35AYMR067CUBX4
YLR039CRIC1YPL115CBEM3YOL059WGPD2YCR095COCA4[YLR185WRPL37A]YBR273CUBX7
YCR028C-ARIM1YDR099WBMH2YDL035CGPR1YDR067COCA6YDR500CRPL37BYDL190CUFD2
YNL069CRPL16BYIL159WBNR1YML121WGTR1YML060WOGG1[YPR043WRPL43A]YNL229CURE2
YDR389WSAC7YFL025CBST1YGL084CGUP1YGR202CPCT1YBR031WRPL4AYKR042WUTH1
YDR143CSAN1YAR014CBUD14YGL237CHAP2[YER178WPDA1]YLR448WRPL6BYDL077CVAM6
YER120WSCS2[YGR262CBUD32b]YBL021CHAP3YER153CPET122YGL147CRPL9AYGL212WVAM7
[YOR035CSHE4]YIL034CCAP2YOR358WHAP5YOR017WPET127YER139CRTR1YIL135CVHS2
YDL033CSLM3YGR174CCBP4YCR065WHCM1YLR191WPEX13YLR180WSAM1YIL017CVID28
YOR327CSNC2YKL208WCBT1YHL002WHSE1YOL044WPEX15YDR181CSAS4YKR001CVPS1
YLR313CSPH1YML036WCGI121cYGL253WHXK2YAL055WPEX22YKL051WSFK1YPL120WVPS30
YLR372WSUR4YIL035CCKA1[YJR118CILM1]YDR329CPEX3YNL032WSIW14YJL154CVPS35
YBR126CTPS1YOR061WCKA2YDR315CIPK1YGR133WPEX4YLR398CSKI2YKR020WVPS51
YBR082CUBC4YOR039WCKB2YFR055WIRC7YGR231CPHB2YPR189WSKI3YDR486CVPS60
YGR072WUPF3YOL008WCOQ10YGL016WKAP122YJL117WPHO86YOR076CSKI7YIL101CXBP1
YOR089CVPS21YPL172CCOX10YHR158CKEL1YCR037CPHO87YGL213CSKI8YIL023CYKE4
YAL002WVPS8YLL009CCOX17YNL238WKEX2YGL023CPIB2YNL311CSKP2YBR104WYMC2
YHR116WCOX23YJL094CKHA1[YML061CPIF1b]YBL007CSLA1YDR349CYPS7
[YMR256CCOX7]YAR018CKIN3YDR466WPKH3YGR229CSMI1[YLR262CYPT6]
YDL117WCYK3YNL322CKRE1YDL095WPMT1YER161CSPT2YML001WYPT7
YOR065WCYT1
  • Underlined genes lie adjacent to other genes whose deletion rescues cdc13-1. In such cases, an effect on transcription of the neighboring gene cannot be discounted. Brackets indicate genes previously identified as synthetic with wild-type during SGA (Tong et al. 2001).

  • a The RTC gene name is reserved for previously unnamed ORFs.

  • b Deletion of PIF1 and BUD32 rescues cdc13-1 (Downey et al. 2006) but these genes were not tested further in robotic assays due to slow growth.

  • c Deletion of CGI121 rescues cdc13-1 (Downey et al. 2006) but this was not detected in robotic assays subsequent to initial scoring.