TABLE 1

Examples to illustrate the interpretation of maternal and progeny genotype data for autosomal markers and the determination of the minimum number of paternal alleles per brood

Example A:Example B:Example C:Example D:
MHco4(WRS) female 2 brood, marker Hcms25MHco4(WRS) female 3 brood, marker Hcms8a20MHco4(WRS) female 4 brood, marker Hcms25MHco4(WRS) female 3 brood, marker Hcms 40
Allele 1Allele 2Inferred paternal allelesAllele 1Allele 2Inferred paternal allelesAllele 1Allele 2Inferred paternal allelesAllele 1Allele 2Inferred paternal alleles
Observed maternal genotypea207213240211
Observed genotype of progenya
    1213211211240248248211213213297297
    2213213 or null240244244215215279279
    3207207 or null240248248213213281281
    4207217217240248248211213213Null
    5213213 or null240244244213213279279
    6213211211240240 or null211211 or null279279
    7213213 or null240244244Null279279
    8207207 or null240240 or null215215281281
    9213209209240240 or null211213213297297
    10207211211240240 or null211211 or nullNull
    11NDNDND240244244211211 or null279279
    12NDNDND240240 or null211211 or nullNull
True maternal genotypeb207213240240211NullNullNull
Paternal alleles present in brood207 or null
209240 or null211 or null279
211244213297
213 or null248215281
217NullNull
Minimum no. of paternal alleles4334
  • Example A: maternal genotype heterozygous for two observed alleles of different size. Example B: maternal genotype homozygous for a single observed allele. Example C: maternal genotype heterozygous for a single observed allele and a null allele. Example D: maternal genotype homozygous for a null allele. —, no alleles amplified from the individual (homozygous for the null allele). ND, not done; only 10 progeny were genotyped from this brood.

  • a Genotypes are based on size of alleles detected by “Genescan.” Where a single allele is observed, this could be either because the individual is homozygous for that allele or alternatively because it is heterozygous for the observed allele and a null allele. Examination of progeny genotypes allows these two possibilities to be discriminated for the maternal genotype.

  • b This is the actual genotype of the mother determined from its observed genotype and taking into account the genotypes of the progeny.