TABLE 1

Rescue of Sxlf7,M1/Sxlf9 mutant ovaries by maternal or zygotic traF

Sxl genotypeExtra copy of snf+?Mode of traF contributionOogenic ovaries per mutant femaleRelative viability
CrossatraF transgene usedMaternalZygoticTwo (%)One (%)None (%)No. females%NREFb
1f7,M1/f9NoNoneNoNo001001511327
2f7,M1/f9NoU2af-traFYesNo0991112732
2f7,M1/f9NoU2af-traFYesYes10000325732
3f7,M1/f9YesNoneNoNo05956553324
4f7,M1/f9YesU2af-traFYesNo2637376561228
4f7,M1/f9YesU2af-traFYesYes95502561216
5f7,M1/f9Yeshsp83-traFYesNo10000206425
6f7,M1/f9YesUAS-traF; no GAL4NoNo00100158537
6f7,M1/f9YesUAS-traF; twi-GAL4NoYes10000157124
7f7,M1/f9YesUAS-traF; no GAL4NoNo02985056273
7f7,M1/f9YesUAS-traF; GAL4-24BNoYes100005043298
  • a Cross 1: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B ☿☿ × ♂♂ y w cm Sxlf9/Y; cross 2: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B; P{U2af50-traF w+mW.hs}2B/+ ☿☿ (sibs to females in 1) × ♂♂ y w cm Sxlf9/Y; cross 3: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B ☿☿ × ♂♂ y w cm Sxlf9/Y; P{snf+ w+mC}19; cross 4: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B; P{U2af50-traF w+mW.hs}2B/+ (sibs to females in 3) ☿☿ × ♂♂ y w cm Sxlf9/Y; P{snf+ w+mC}19; cross 5: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B; P{hsp83-traF w+mC}5.4/+ ☿☿ × ♂♂ y w cm Sxlf9/Y; P{snf+ w+mC}19; cross 6: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B; CyO, P{GAL4-twi.G w+mC }2.2, P{UAS-2xEGFP}AH2.2/+ ☿☿ × ♂♂ y w cm Sxlf9/Y; P{snf+ w+mC}19 P{UAS-traF w+mC}20J7; cross 7: w cm Sxlf7,M1 ct v/Binsinscy, y w snx2B; P{hsp70-GAL4 w+mW}24B/+ ☿☿ × ♂♂ y w cm Sxlf9/Y; P{snf+ w+mC}19 P{UAS-traF w+mC}20J7.

  • b Binsinscy/Sxlf9 siblings with the same autosomal genotype as experimentals were the viability reference.