Genetic screen statistics

No. of mutant lines% of previous round
Total mutants tested1452
    P-element lines1045
    EMS lines407
First-round positives704.8
    P-element lines605.7
    EMS lines102.5
Second-round positives2333
    P-element lines1830
    EMS lines550
Final-round positives1879
    P-element lines1478
    EMS lines480
  • The selection of mutants through three rounds of screening is shown. It demonstrates a purification effect in which the percentage of mutants reisolated during each round rises from ∼5% in the first round to ∼80% in the final round.