TABLE 2

Plasmids

NameDescription
pCLW18pRS316 SIR2 (URA3, CEN6, its own promoter)
pCLW19pRS316 sir2-276 (URA3, CEN6, its own promoter)
pCLW21pRS313 SIR2 (HIS3, CEN6, its own promoter)
pCLW22pRS316 sir2-276 (HIS3, CEN6, its own promoter)
pDM111apGEX-5X-1 SIR2 (GST-SIR2)
pCLW20pGEX-5X-1 sir2-276 (GST-sir2-276)
pJWL03pET28b HST2 (His6-HST2)
pJWL41pET28b hst2 R45A (His6-hst2 R45A)
pJWL43pET28b hst2 H135A (His6-hst2 H135A)
pJWL46pET28b hst2 Q115A (His6-hst2 Q115A)
pJWL47pET28b hst2 E128A (His6-hst2 E128A)