TABLE 2

Plasmid constructs used in this study

ConstructDescription
pCDC3DNA fragment encoding Cdc3p (1–520 aa) was cloned into pGBKT7.
pCDC10DNA fragment encoding Cdc10p (1–322 aa) was cloned into pGBKT7.
pCDC11DNA fragment encoding Cdc11p (1–415 aa) was cloned into pGBKT7.
pCDC12DNA fragment encoding Cdc12p (1–407 aa) was cloned into pGBKT7.
pSYP1DNA fragment encoding Syp1p (1–870 aa) was cloned into pGADT7.
pGEX-CDC3DNA fragment encoding Cdc3p (1–520 aa) was cloned into pGEX-4T-1.
pGEX-CDC10DNA fragment encoding Cdc10p (1–322 aa) was cloned into pGEX-4T-1.
pGEX-CDC11DNA fragment encoding Cdc11p (1–415 aa) was cloned into pGEX-4T-1.
pGEX-CDC12DNA fragment encoding Cdc12p (1–407 aa) was cloned into pGEX-4T-1.
pGAL-SYP1-HA-314DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal HA epitope followed by the ADH1 terminator and placed under GAL1 promoter control in pRS314.
pGAL-SYP1-HA-306DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal HA epitope followed by the ADH1 terminator and placed under GAL1 promoter control in pRS306.
pGAL-SYP1-Myc-306DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal Myc epitope followed by the ADH1 terminator and placed under GAL1 promoter control in pRS306.
pSWE1-HA-314DNA fragment encoding Swe1p (1–819 aa) was cloned in frame with a C-terminal HA epitope followed by the ADH1 terminator and placed under SWE1 promoter control in pRS314.
pCDC12-GFP-305DNA fragment encoding Cdc12p (1–407 aa) was cloned in frame with a C-terminal GFP epitope followed by the ADH1 terminator in pRS305.
pCDC12-YFP-304DNA fragment encoding Cdc12p (1–407 aa) was cloned in frame with a C-terminal YFP epitope followed by the ADH1 terminator in pRS304.
pCDC10-GFP-305DNA fragment encoding Cdc10p (1–322 aa) was cloned in frame with a C-terminal GFP epitope followed by the ADH1 terminator in pRS305.
pSYP1-CFP-306DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal CFP epitope followed by the ADH1 terminator in pRS306.
pSYP1-HA-306DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal HA epitope followed by the ADH1 terminator in pRS306.
pSYP1-GFP-305DNA fragment encoding Syp1p (1–870 aa) was cloned in frame with a C-terminal HA epitope followed by the ADH1 terminator in pRS305.