TABLE 1

Yeast strains used in this study

StrainGenotype
W303-1AMATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1
SFY526MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3,112 canr gal4-542 gal80-538 URA3∷GAL1-lacZ
YMC515QMATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 syp1Δ∷HIS3
YMC516QMATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 syp1Δ∷HIS3 cdc12∷CDC12-GFP-LEU2
YMC517MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc12∷CDC12-GFP-LEU2
YMC518MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc12∷CDC12-GFP-LEU2/p314
YMC519MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc12∷CDC12-GFP-LEU2/pGAL-SYP1-HA-314
YMC520MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1∷GAL-HA-URA
YMC521MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1∷GAL-SYP1-HA-URA
US810MATα ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 swe1Δ∷HIS3
YMC522MATα ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 swe1Δ∷HIS3∷GAL-HA-URA
YMC523MATα ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 swe1Δ∷HIS3∷GAL-SYP1-HA-URA
YMC524MATα ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 swe1Δ∷HIS3∷GAL-SYP1-Myc-URA/p314
YMC525MATα ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 swe1Δ∷HIS3∷GAL-SYP1-Myc-URA/pSWE1-HA-314
YMC526MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc10∷CDC10-GFP-LEU2∷GAL-HA-URA
YMC527MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc10∷CDC10-GFP-LEU2∷GAL-SYP1-HA-URA
YEF473-1619MATα his3 leu2 lys2 ura3 trp1cdc10-1
YMC528MATα his3 leu2 lys2 ura3 trp1cdc10-1∷cdc12∷CDC12-GFP-LEU∷GAL-HA-URA
YMC529MATα his3 leu2 lys2 ura3 trp1cdc10-1∷cdc12∷CDC12-GFP-LEU∷GAL-SYP1-HA-URA
YMC530MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 cdc12∷CDC12-YFP-TRP syp1∷SYP1-CFP-URA
YMC531MATα his3 leu2 lys2 ura3 trp1cdc10-1 cdc12∷CDC12-GFP-LEU
YMC532MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 syp1∷SYP1-HA-URA
YMC533MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 syp1∷SYP1-GFP-LEU
YMC534MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3-1 syp1∷SYP1-CFP-URA
YMC535MATα his3 leu2 lys2 ura3 trp1cdc10-1 cdc12∷SYP1-CFP-URA
YMC536MATα his3 leu2 lys2 ura3 trp1rts1Δ∷HIS
YMC537MATα his3 leu2 lys2 ura3 trp1rts1Δ∷HIS syp1Δ∷URA