TABLE 3

Proteins identified as being differentially expressed in wild-type and Ocd flies

MW (kDa)pI (pH)Corresponding polypeptide(s)FunctionP score from nested ANOVARatio change
A. Nuclear-encoded mitochondrial genes
58.09.1CG3861-PBCitrate synthase0.0026+1.57
58.39.1CG3861-PA CG3861-PB0.0266+1.40
59.09.1CG3612-PAATP synthase α- subunit (Bellwether)0.00426+1.39
59.69.1CG3612-PA0.0147+1.53
59.69.1CG3612-PA0.0204+1.25
59.69.1CG3612-PA0.0241+1.45
59.69.1CG3612-PA0.04951−1.23
86.28.5CG9244-PBAconitase0.01539+1.40
54.08.6CG4094-PA CG4094-PBFumarate hydratase0.0171+1.28
41.08.8CG6439-PAIsocitrate dehydrogenase β- subunit0.0381+1.26
B. Nonmitochondrial genes
44.65.9EnolaseEnolase (Drosophila pseudobscura)0.0077−1.20
41.06.3CG6663-PAPeptidase inhibitor homolog0.0104+1.65
74.39.2CG5939-PCParamyosin0.0241+1.45
61.76.1CG6148-PA CG6148-PBPutative achaete scute target 10.0270+1.30
  • Significant differences in protein abundance are associated with ANOVA P-scores of <0.05. Molecular weights (MW) and isoelectric points (pI) are shown. Each entry in the MW column indicates a spot independently identified. Polypeptides are named according to FlyBase nomenclature. In several cases different spots correspond to the same polypeptide which, due to post-translational modification, migrates to a different position on the gel. Proteins were identified by searching the NCBI database. The corresponding polypeptides and encoding genes are listed. The last two columns list the nested ANOVA P-values and the ration change. Positive values indicate proteins more abundant in Ocd flies; negative values indicate those more abundant in wild type.