TABLE 3

glo-3(−) double mutants

% of animals with the specified no. of autofluorescent granules in intestinal cells
GenotypeNoneLowMediumHighn
Wild type00010024
glo-3(zu446)a0955085
glo-1(zu437)10000041
glo-4(ok623)b10000032
pgp-2(kx48)c0397035
apt-6(ok429)16840019
glo-1(zu437) glo-3(zu446)10000049
glo-4(ok623); glo-3(zu446)10000095
pgp-2(kx48); glo-3(zu446)10000054
apt-6(ok429); glo-3(zu446)9190031
  • All strains were grown at 22°. Individual L4/young adults were analyzed using fluorescence microscopy with a fluorescein isothiocyanate (FITC) filter and were scored for the number of autofluorescent gut granules within the intestine. Animals were scored as none when autofluorescent gut granules were lacking, as low when 1–100 autofluorescent gut granules were present, as medium when 101–200 autofluorescent gut granules were present, and as high when >200 autofluorescent gut granules were present.

  • a The linked markers egl-15(n484) or unc-27(e159) did not alter the Glo phenotype of glo-3(zu446). n, number of animals scored.

  • b The linked marker dpy-11(e224) did not alter the Glo phenotype of glo-4(ok623).

  • c The linked marker dpy-5(e61) did not alter the Glo phenotype of pgp-2(kx48).