TABLE 2

Autofluorescent gut granules in glo-3(−) adults

% of animals with the specified no. of autofluorescent granules in anterior intestinal cells% of animals with the specified no. of autofluorescent granules in posterior intestinal cellsPhenotype (anterior/posterior)
GenotypeVery lowLowMediumHighVery lowLowMediumHighn
Wild type00010000199High/high89
glo-3(RNAi)a01000008550Low/low40
Class I alleles
    glo-3(kx91)100000168400Very low/low109
    glo-3(kx94)9640088840Very low/low77
Class II alleles
    glo-3(gm125)19630010000Low/low77
    glo-3(kx29)23770009910Low/low86
    glo-3(kx37)52480009190Low/low67
    glo-3(kx90)23770009640Low/low74
    glo-3(zu446)336700186130Low/low95
Class III alleles
    glo-3(kx1)283123050455Low/low58
    glo-3(kx38)010000079210Low/low71
  • All strains were grown at 22°. Individual young adults were analyzed using fluorescence microscopy with a fluorescein isothiocyanate (FITC) filter and were scored for the number of autofluorescent gut granules within the anterior and posterior areas of the intestine. Young animals were scored as very low when 0–5 autofluorescent gut granules were present, as low when 6–100 autofluorescent gut granules were present, as medium when 101–200 autofluorescent gut granules were present, and as high when >200 autofluorescent gut granules were present. n, number of animals scored.

  • a RNAi was carried out in the rrf-3(pk1426) RNAi-sensitive background (Simmer et al. 2003). Similar results were seen in the N2 strain.