TABLE 1

Birefringent gut granules in glo-3(−) embryos

Genotype% of embryos that lacked birefringence in intestinal cells (% of embryos that also mislocalized birefringent material into the intestinal lumen)% of embryos with 1–10 enlarged birefringent granules in intestinal cells (% of embryos that also mislocalized birefringent material into the intestinal lumen)% of embryos with 11–30 enlarged birefringent granules in intestinal cells (% of embryos that also mislocalized birefringent material into the intestinal lumen)n
Wild typea00052
Class I alleles0
    glo-3(kx91)100 (47)0077
    glo-3(kx94)100 (56)00107
Class II alleles0
    glo-3(gm125)41 (40)59 (23)083
    glo-3(kx29)41 (29)59 (23)073
    glo-3(kx37)68 (38)32 (22)069
    glo-3(kx90)47 (26)53 (23)0115
    glo-3(zu446)54 (38)46 (15)0125
Class III alleles
    glo-3(kx1)012 (0)88 (0)91
    glo-3(kx38)025 (5)75 (0)92
smg-1(−) suppression
smg-1(r861)b002 (0)54
Class I alleles
    glo-3(kx91); smg-1(r861)100 (94)0085
    glo-3(kx94); smg-1(r861)100 (63)0068
Class II alleles
    glo-3(gm125); smg-1(r861)00100 (0)26
    glo-3(kx29); smg-1(r861)00100 (0)41
    glo-3(kx37); smg-1(r861)03 (100)97 (0)59
    glo-3(kx90); smg-1(r861)00100 (0)24
    glo-3(zu446); smg-1(r861)068 (6)32 (0)34
Class III alleles
    glo-3(kx1); smg-1(r861)00100 (0)27
    glo-3(kx38); smg-1(r861)00100 (0)28
Parental genotypec
Class I alleles
    glo-3(kx91)/+15 (9)19 (11)0186
    glo-3(kx94)/+9 (4)15 (9)0110
Class II alleles
    glo-3(kx90)/+10 (6)12 (6)0177
    glo-3(zu446)/+11 (4)14 (3)0109
  • All strains were grown at 22°. Twofold and later-stage embryos were analyzed using polarization microscopy and scored for the presence, morphology, and localization of birefringent material in the intestine. n, number of embryos scored.

  • a Wild-type embryos contained >100 birefringent granules in intestinal cells and lacked birefringent material in the intestinal lumen.

  • b Ninety-eight percent of smg-1(r861) embryos contained >100 birefringent granules in intestinal cells and lacked birefringent material in the intestinal lumen.

  • c The progeny of heterozygous glo-3(−)/+ hermaphrodites were scored. Twenty-five percent are predicted to be glo-3(−)/glo-3(−) and exhibit a Glo phenotype.