dArf6KO targeting with and without using UAS-Rpr as a negative selection marker

Screening cross set upProgenies screenedMale candidatesSecond chr candidatesRpr+FRT+PCR verified
(×) w/Y; Pin/CyO; Gal4221[w−]>7 × 10531530/315ND5/30
(×) w/Y; Pin/CyO∼73001242a/124122/12454/124ND
  • chr, chromosome; ND, not done; —, not applicable.

  • a These two candidates harbored nontargeted integration of donor DNA on the second chromosome and were Rpr+.