Footprints identified in homozygous unc-5(ox171) and unc-22(kr5) backgrounds

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  • Footprints are represented and classified according to the rules defined by Robert and Bessereau (2007). Briefly, class I events are typical end-joining footprints. In subclass Ia footprints, it is possible to recognize the TA dinucleotide duplicated during Mos1 insertion and some Mos1 sequences left during excision. In subclass Ib footprints, at least one copy of the duplicated TA dinucleotide is missing, as expected if processing of the ends of the break occurs before ligation. Class III footprints contain duplication of the genomic sequences adjacent to the broken site which might result from intrachromosomal recombination (see Figure S1 in Robert and Bessereau 2007).