Reversion efficiencies at the unc-5 and unc-22 loci

TransposaseHomozygous backgroundWild-type/total footprintsNo. of experimentsNo.of transgenesHeterozygous backgroundWild-type/total footprintsNo. of experimentsNo. of transgenes
    hsp-16.48∷Tpase (HS+)10/40,000 = 2.5 × 10−42/15167432/13,000 = 3.3 × 10−252/52158
    No Tpase (HS+)0/12,000 < 8.3 × 10−5750/16,500 < 6 × 10−555
    glh-2∷Tpase (HS−)3/60,000 = 5 × 10−56/18314/2700 = 1.4 × 10−34/411
    vit-2∷Tpase (HS−)ND222/8300 = 2.4 × 10−42/283
    hsp-16.48∷Tpase (HS+)1/4000 = 2.5 × 10−41/33111/700 = 1.6 × 10−210/1021
    hsp-16.48∷Tpase (HS−)ND0/650 < 1.5 × 10−311
  • Reversion experiments were performed in homozygous backgrounds (respectively, ox171∷Mos1/ox171∷Mos1 and kr5∷Mos1/kr5∷Mos1) and in heterozygous backgrounds (respectively, e184sd ox171∷Mos1/idDf3 and kr5∷Mos1/st136∷Tc1). Reversion efficiences were calculated as the ratio between the number of phenotypic revertants and the total number of screened animals. Mos1 excision was triggered by expression of the Mos transposase from different transgenes. hsp-16.48 is a heat-shock-inducible promoter. Reversion assays were performed by heat-shocking parent animals and screening the progeny for revertants as described in Robert and Bessereau (2007). When working with transgenes expressing the Mos transposase under the control of the glh-2 or vit-2 promoters, transgenic L4's were selected and grown at 20° for 5 days before screening their progeny for revertants. “HS+” or “HS−” indicate whether or not parent animals were heat-shocked. “Wild-type/total footprints” indicates the number of wild-type footprints identified among the population analyzed by sequencing. Footprints identified in homozygous backgrounds are listed in Table 2. “No. of experiments” refers to the number of independent experiments. “No. of transgenes” indicates the number of independent trangenes used to express the transposase.