TABLE 4

Average length of cell cycle in cells undergoing mating-type switching

Average time required for cell division (hr)
UninducedTwo switchedOne switched, one deadTwo dead
Wild type4.5 ± 0.24.5 ± 0.1NANA
donorless4.9 ± 0.2NANA10 ± 0.2*
rad1Δ4.7 ± 0.27.0 ± 0.3*8.4 ± 0.3*8.2 ± 0.4*
slx4Δ4.7 ± 0.15.8 ± 0.2*7.0 ± 0.4*8.6 ± 0.4*
rad1Δ slx4Δ4.5 ± 0.15.5 ± 0.4*6.8 ± 0.4*9.0 ± 0.4*
msh2Δ4.3 ± 0.15.6 ± 0.1*NA6.5 ± 0.3*
rad9Δ4.9 ± 0.14.5 ± 0.14.8 ± 0.2NA
mad2Δ4.9 ± 0.24.9 ± 0.24.2 ± 0.3NA
rad1Δ rad9Δ5.2 ± 0.1*4.8 ± 0.24.8 ± 0.15.3 ± 0.1
rad1Δ mad2Δ5.5 ± 0.2*5.6 ± 0.3*6.7 ± 0.3*6.3 ± 0.2*
mms2Δ4.5 ± 0.15.1 ± 0.1*NA6.1 ± 0.5*
mph1Δ4.5 ± 0.14.7 ± 0.15.9 ± 0.6*6.5 ± 0.8*
rad1Δ mms2Δ4.5 ± 0.15.8 ± 0.3*6.4 ± 0.1*6.3 ± 0.1*
rad1Δ mph1Δ5.3 ± 0.1*6.2 ± 0.3*8.1 ± 0.3*8.3 ± 0.3*
  • Cell cycle duration was determined during pedigree analysis (see Table 3 and materials and methods) and is shown as the mean length of time required for division (hr) ± SEM. “NA” denotes categories containing <10% of cells, and thus cell cycle lengths are not reported. Asterisks denote statistical significance from wild type with P < 0.01; values were compared to wild type “uninduced” or “two switched” as appropriate.