TABLE 3

Pedigree analysis of wild type and mutants induced for mating-type switching (MATa to MATα) or mock induced

% two switched% one switched, one dead% two dead% otherN
A. Induced
Wild type9640081
donorless0096451
rad1Δ383228297
slx4Δ513312449
rad1Δ slx4Δ294419859
msh2Δ78711481
rad9Δ82126090
mad2Δ84104249
rad1Δ rad9Δ2041390102
rad1Δ mad2Δ334315860
mms2Δ79712258
mph1Δ691610570
rad1Δ mms2Δ195426199
rad1Δ mph1Δ283830381
% two alive% one alive, one dead% two deadN
B. Mock induced
Wild type990191
donorless1000041
rad1Δ9820119
slx4Δ943336
rad1Δ slx4Δ945262
msh2Δ849776
rad9Δ980251
mad2Δ1000026
rad1Δ rad9Δ893872
rad1Δ mad2Δ912853
mms2Δ907371
mph1Δ1000052
rad1Δ mms2Δ879492
rad1Δ mph1Δ963175
  • Following induction of mating-type switching in liquid culture, single cells were separated on YPD medium under the light microscope using a microdissection needle. Cells were monitored at regular intervals, and daughter cells were separated after completion of the first cell division. Cells that formed colonies were tested for mating type as described in materials and methods. The number of cells (N) tested for each strain is shown. For cells induced for mating-type switching, “% other” includes pairs of daughter cells scored as “one unswitched and one dead,” or “one unswitched and one switched.” Pairs of daughter cells scored as “both unswitched” were not included because we cannot rule out the failure to form a DSB in these cells. For the uninduced experiments, viability is shown for cells mock induced with water.