TABLE 1

Strains used in this study

NameGenotypeStrain notes and deletion constructs
MATa to MATα
EAY745Wild typeDerived from JKM161 (Δho, HMLα, MATa, Δhmr∷ADE1, ade1-100, leu2-3,112, lys5, trp1∷hisG, ura3-52, ade3∷GAL10∷HO)
EAY853rad1ΔpWS1510 (rad1Δ∷URA3; E. Friedberg)
EAY969msh2ΔpEAI99 (msh2Δ∷TRP1; this lab)
EAY854msh3ΔpEAI88 (msh3Δ∷hisG-URA3-hisG; this lab)
EAY2087slx4Δslx4Δ∷KANMX
EAY1788pol3-01YIpAM26 (pol3-01∷URA3; from A. Sugino)
EAY1332mus81Δmus81Δ∷KANMX
EAY1730rad9Δrad9Δ∷KANMX
EAY1968mad2Δmad2Δ∷KANMX
EAY1562mms2Δmms2Δ∷KANMX
EAY1778mph1Δmph1Δ∷KANMX
EAY2125rad1Δrad10Δrad10Δ∷KANMX; see above
EAY2090rad1Δslx4ΔSee above
EAY1803rad1Δ pol3-01See above
EAY797rad1Δmus81ΔSee above
EAY1726rad1Δrad9ΔSee above
EAY1973rad1Δ mad2ΔSee above
EAY1725rad1Δmms2ΔSee above
EAY1776rad1Δ mph1ΔSee above
MATa to MATa
EAY744Wild typeDerived from JKM160 (Δho, Δhml∷ADE1, MATa, HMRa, ade1-100, leu2-3,112, lys5, trp1∷hisG, ura3-52, ade3∷GAL10∷HO)
EAY1356rad1ΔpWS1510 (rad1Δ∷URA3; E. Friedberg)
EAY2084slx4Δslx4Δ∷KANMX
MATaKANMX to MATα
EAY1042Wild typeDerived from JKM161; see materials and methods
EAY1115rad1ΔpWS1510 (rad1Δ∷URA3; E. Friedberg)
EAY1040msh2ΔpEAI99 (msh2Δ∷TRP1)
EAY1118msh3ΔpEAI88 (msh3Δ∷hisG-URA3-hisG)
EAY1407rad51ΔpJH683 (rad51Δ∷URA3; from J. Haber)
donorless
EAY742Wild typeDerived from JKM139 (Δho, Δhml∷ADE1, MATa, Δhmr∷ADE1, ade1-100, leu2-3,112, lys5, trp1∷hisG, ura3-52, ade3∷GAL10∷HO)
  • All strains used in this study are derived from JKM161, JKM160, and JKM139, kindly provided by J. Haber. Gene disruptions and mutant alleles were made by transforming S. cerevisiae strains with restriction-digested plasmids for rad1Δ, msh2Δ, msh3Δ, rad51Δ, and pol3-01 as listed above. All other disruptions were made by integrative transformation of PCR products generated by amplification of KANMX sequences from either plasmid pFA6-KanMX4 (Wach et al. 1994) or genomic DNA from the Saccharomyces Genome Deletion Project knockout strains (http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html). See materials and methods for details.