TABLE 1

Genetic map distances and distribution of parental and recombinant progeny in wild-type, mlh3Δ, mlh3-D523N, mms4Δ, mlh3Δ mms4Δ, and mlh3-D523N mms4Δ strains

Single sporesTetrads
GenotypenParental single sporesRecombinant single sporescM95% C.I.nPDTTNPDcMSE
URA3-LEU2
Wild typea46443635100921.720.6–23.01068607456522.821.8–23.8
mlh3Δ3023268234111.310.2–12.5582460114813.912.3–15.5
mlh3-D523N3059272733210.99.8–12.0575455117311.710.5–12.9
mms4Δa2732222750518.517.1–20.015310251016.714.8–18.6
mlh3Δ mms4Δ21272107200.900.59–1.5234229501.10.6–1.5
mlh3-D523N mms4Δ1156115330.300.07–0.82152152000.00.0
LEU2-LYS2
Wild typea46443388125627.025.8–28.41068496569327.526.6–28.4
mlh3Δ3023261041313.712.5–15.0582424154415.313.9–16.6
mlh3-D523N3059265240713.312.1–14.6575417155315.013.8–16.3
mms4Δa2732208165123.822.3–25.51537775126.523.8–29.2
mlh3Δ mms4Δ21272073542.51.9–3.32342231102.41.7–3.0
mlh3-D523N mms4Δ11561135211.81.2–2.8152147501.60.92–2.4
LYS2-ADE2
Wild typea4644405259212.711.8–13.81068803263212.912.1–13.7
mlh3Δ302328222016.65.8–7.658250181076.2–7.7
mlh3-D523N305928801795.95.0–6.85755126215.95.1–6.7
mms4Δa2732244728510.49.3–11.71531242909.57.9–11.1
mlh3Δ mms4Δ21272101261.20.81–1.8234228601.30.76–1.8
mlh3-D523N mms4Δ11561142141.20.69–2.0152149301.00.43–1.5
ADE2-HIS3
Wild typea46443033161134.733.3–36.110683437091637.736.5–38.9
mlh3Δ3023248553817.81.6–1.9582379201218.317.1–19.5
mlh3-D523N3059251254717.91.6–1.9575373197519.718.3–21.2
mms4Δa2732192380929.627.9–31.41535994030.728.7–32.7
mlh3Δ mms4Δ21272053743.52.8–4.42342161803.83.0–4.7
mlh3-D523N mms4Δ11561119373.22.3–4.41521421003.32.3–4.3
URA3-LYS2
Wild typea46442815182939.438.0–40.810682647594548.246.5–49.9
mlh3Δ3023235966422.020.5–23.5582333240925.323.5–27.0
mlh3-D523N3059239066921.920.4–23.45753272381025.924.1–27.7
mms4Δa2732176496835.433.6–37.31534998643.839.2–48.4
mlh3Δ mms4Δ21272059683.22.5–4.02342191503.22.4–4.0
mlh3-D523N mms4Δ11561134221.91.2–2.9152147501.60.92–2.4
LYS2-HIS3
Wild typea46442829181539.137.7–40.510682787444647.846.0–49.6
mlh3Δ3023236066321.920.5–23.5582333240925.323.5–27.0
mlh3-D523N3059239366621.820.3–23.3575334232924.923.1–26.6
mms4Δa2732180692633.932.1–35.71535398235.932.8–39.0
mlh3Δ mms4Δ21272029984.63.8–5.62342102405.14.1–6.1
mlh3-D523N mms4Δ11561107494.23.2–5.61521391304.33.1–5.4
  • All mutants are isogenic derivatives of EAY1108/EAY1112 (materials and methods). For single spores, the recombination frequencies (number of recombinant spores/total spores) were multiplied by 100 to yield genetic map distances (in centimorgans); 95% confidence intervals for genetic map distance in the single spores were determined (http://faculty.vassar.edu/lowry/VassarStats.html). For tetrads, genetic distance in centimorgans (cM) was calculated using the formula of Perkins (1949): 50 × {TT + (6 × NPD)}/(PD + TT + NPD). For tetrad data, +/− 1 standard error around the genetic distance was calculated using the Stahl Laboratory Online Tools website (http://www.molbio.uoregon.edu/∼fstahl/). n, number of single spores or tetrads analyzed; PD, parental ditype; TT, tetratype; NPD, nonparental ditype.

  • a Data are from Argueso et al. (2004).