TABLE 1

List of strains

StrainGenotypeSource
W303MATa leu2-3,112 ura3-1 trp1-1 his3-11,15 can1-100 GAL SUC2S. Lindquist
hsc82ΔIsogenic to W303 except hsc82Δ∷LEU2A. Duina
CMY8015Isogenic to W303 except STI1/MycThis study
CMY1200Isogenic to hsc82Δ except HSP82/MycThis study
CMY8001Isogenic to CMY1200 except sti1Δ∷HygRThis study
S153MATa leu2-3,112 ura3-1 trp1-1 ade2-1 can1-100 GAL SUC hsc82Δ∷LEU2 hsp82Δ∷LEU2 pGPD-hsp82-T1011D. Nathan
cpr7ΔIsogenic to W303 except cpr7Δ∷HIS3A. Duina
hsc82Δ cpr7ΔIsogenic to W303 except hsc82Δ∷LEU2 cpr7Δ∷TRP1A. Duina
5CG2MATα ura3-52 lys2-801 ade2-101 trp1-63 his3-200 leu2-1 hsc82∷URA3 hsp82∷GAL1-HSP82∷LEU2S. Lindquist
JN516MATa ura3-52 leu2-3,112 his3-11 trp1Δ1 lys2 SSA1 ssa2∷LEU2 ssa3∷TRP1 ssa4∷LYS2 hem1-Δ100E. Craig and L. Zhang
CMY1300Isogenic to JN516 except SSA1/MycThis study
CMY8002Isogenic to JN516 except sti1Δ∷HygRThis study
5B6MATa ura3-52 leu2-3,112 his3-11 trp1Δ1 lys2 ssa1∷HIS3 ssa2∷LEU2 SSA3 ssa4∷LYS2 hem1-Δ100 pGAL1-SSA1E. Craig and L. Zhang