TABLE 3

Chromatin immunoprecipitation of endogenous sequences in cis to bwD

AntibodyPrimer setcsc2/+acsc2/bwDabcRatio bwD:+bcd
H3bw (exon)0.31 ± 0.0030.34 ± 0.0091.10
bw (intron)0.34 ± 0.0100.34 ± 0.0201.00
cg301810.35 ± 0.0150.34 ± 0.0070.97
hsp70-mw0.33 ± 0.0150.31 ± 0.0120.94
AcH3bw (exon)0.34 ± 0.0190.33 ± 0.009 0.30 ± 0.0090.97 0.87
bw (intron)0.40 ± 0.0030.30 ± 0.0090.75
cg301810.41 ± 0.0120.32 ± 0.0270.78
hsp70-mw0.35 ± 0.0340.27 ± 0.0300.77
2MeH3K9bw (exon)0.24 ± 0.0220.38 ± 0.035 0.34 ± 0.0321.58 1.42
bw (intron)0.34 ± 0.0320.32 ± 0.0380.94
cg301810.31 ± 0.0150.32 ± 0.0091.03
hsp70-mw0.31 ± 0.0300.35 ± 0.0541.13
HP1bw (exon)0.21 ± 0.0310.38 ± 0.0261.81
bw (intron)0.21 ± 0.0170.35 ± 0.0201.67
cg301810.23 ± 0.0120.38 ± 0.0201.65
hsp70-mw0.31 ± 0.0220.34 ± 0.0351.10
  • a Amount of genomic sequence precipitated by the antibody and then PCR amplified by the primers near bw relative to those PCR amplified by a control primer on another chromosome arm. ±1 standard error.

  • b Italics indicate that csc2/bwD values differ significantly (P-value < 0.05) from csc2/+.

  • c Underlined values are normalized to H3 where appropriate; i.e., H3 occupancy is significantly different and the antibody recognizes part of a histone.

  • d Ratios >1 indicate an increase in that mark or protein on the chromatin amplified relative to the nonheterochromatic genotype; those <1 indicate a decrease.