TABLE 2

Chromatin immunoprecipitation of mini-white (mw) over bwD

AdultLarvae
AntibodyPrimer setmw/+amw/bwDabRatio bwD:+bcmw/+amw/bwDabRatio bwD:+bc
H3hsp70-mw0.54 ± 0.0520.46 ± 0.0520.850.52 ± 0.0150.48 ± 0.0150.92
mw(reg)0.55 ± 0.0410.45 ± 0.0410.820.47 ± 0.0380.53 ± 0.0381.13
AcH3hsp70-mw0.57 ± 0.0420.43 ± 0.0420.750.48 ± 0.0170.52 ± 0.0171.08
mw(reg)0.55 ± 0.0170.45 ± 0.0170.820.49 ± 0.0320.51 ± 0.0321.04
2MeH3K9hsp70-mw0.49 ± 0.0090.51 ± 0.0091.040.50 ± 0.0120.50 ± 0.0121.00
mw(reg)0.51 ± 0.0240.49 ± 0.0240.960.50 ± 0.0090.50 ± 0.0091.00
HP1hsp70-mw0.45 ± 0.0170.55 ± 0.0171.220.47 ± 0.0230.53 ± 0.0231.13
mw(reg)0.41 ± 0.0330.59 ± 0.0331.440.47 ± 0.0090.53 ± 0.0091.13
  • a Amount of genomic sequence precipitated by the antibody and then PCR amplified by the primers near bw relative to those PCR amplified by a control primer on another chromosome arm. ±1 standard error.

  • b Italics indicate that csc/bwD values differ significantly (P-value <0.05) from csc2/+.

  • c Ratios >1 indicate an increase in that mark or protein on the chromatin amplified relative to the non-heterochromatic genotype, those 1, a decrease.