TABLE 4

Characterization of Drosophila mutants—sensitivity to S. aureus and S. typhimurium and growth of L. monocytogenes

PBac lineCG no.SymbolS. aureusS. typhimuriumBacterial load
10067CG10536cbx+0+
10675CG15120/CG16926+0+
10881CG32706+0+
17872CG4857+0+a
18050CG2247+0+
19207CG2397cyp6a13+0+b
19220CG3056+0+
10036CG11489+0+
18609CG12487bobA+0+b
18270CG3527+00
18398CG2152pcmt+00
19305CG7408+00
10496CG1489900+
19181CG612800+
18678CG2914ets21C000
10269CG13564000
10618CG11293++0
10743CG13788gr28b++
  • Mutant lines were injected with 102 CFUs of S. aureus or 104 CFUs of S. typhimurium and the survival of the flies was monitored over the course of the infection. The growth of L. monocytogenes in mutant lines was determined as described in materials and methods. + indicates increased growth or sensitivity compared to wild-type flies, − indicates resistance or decreased growth compared to wild-type flies. 0 indicates no change in sensitivity or growth compared to wild type. Statistical analysis on survival curves was done using log-rank analysis and lines with P < 0.05 were considered statistically significant. Statistical analysis on bacterial growth was done using a t-test and lines with P < 0.05 were considered statistically significant.

  • a Increased growth at 24 hr postinfection only.

  • b Increased growth at 48 hr postinfection only.