TABLE 3

Impact on genome stability due to deletions in COMA complex genes

COMA mutationStrain backgroundSectoring (%)Chromosome V instability rate (events/cell/generation)Fold change over parental strain
Wild type0.077.86E-06
rad9Δ/rad9Δ0.273.37E-05
mcm21Δ/MCM21Wild type4.104.03E-03512.72
mcm21Δ/MCM21rad9Δ/rad9Δ0.654.21E-060.12
mcm21Δ/mcm21ΔWild type0.001.94E-022468.19
mcm21Δ/mcm21Δrad9Δ/rad9Δ0.001.87E-02554.90
ctf19Δ/CTF19Wild type6.802.54E-0432.32
ctf19Δ/CTF19rad9Δ/rad9Δ8.601.64E-044.87
ctf19Δ/ctf19ΔWild type0.001.81E-022302.80
ctf19Δ/ctf19Δrad9Δ/rad9Δ0.001.64E-02486.65
okp1Δ/OKP1Wild type5.002.72E-053.46
okp1Δ/OKP1rad9Δ/rad9Δ3.331.29E-060.04
ame1Δ/AME1Wild type6.601.47E-051.87
ame1Δ/AME1rad9Δ/rad9Δ6.603.08E-050.91
  • Sectoring and chromosome V instability rates are shown for heterozygous and homozygous deletion strains for the four members of the COMA complex. Sectoring is shown as a percentage of the total number of sectors in ∼500 colonies. Chromosome V instability rate (events/cell/generation) as measured by conversion to canavanine-resistant cells was estimated via fluctuation analysis. The fold change in the chromosome V instability rate for the indicated strain compared to the respective parental strain rate is shown in column 4. OKP1 and AME1 are essential genes and data are available only for heterozygous strains.