TABLE 1

Chromosome V instability rate of mutant strains for 65 genes identified in the screen

GOIMutation rate (μ) of insertional mutantμ of precise deletion (rad9Δ/rad9Δ, goiΔ/GOI)Ratio of μ (rad9Δ/rad9Δ goiΔ/GOI) to μ (ISM)A: ratio of μ (rad9Δ/rad9Δ goiΔ/GOI) to μ (rad9Δ/rad9Δ)μ (goiΔ/GOI)B: ratio of μ (goiΔ/GOI) to μ (wild type)A:B ratio
Wild type7.86E-06
rad9Δ/rad9Δ3.37E-05
SAM1 (A)4.36E-042.12E-040.496.298.67E-061.15.72
TMA17 (B)1.15E-049.87E-050.862.936.90E-060.883.33
ARO4 (A)9.56E-055.14E-050.541.533.99E-060.513
DML1 (A)7.59E-051.35E-041.784.011.08E-051.372.93
MSH5 (A)4.01E-051.79E-044.465.322.48E-053.161.68
YBL104C (B)1.91E-048.17E-050.432.421.13E-051.441.68
DPB11 (A)2.67E-051.69E-046.3352.54E-053.231.55
SPP2 (C)6.33E-042.07E-040.336.133.33E-054.241.45
CHS3 (A)6.06E-058.19E-051.352.431.51E-051.921.27
TUB2 (A)5.73E-052.85E-044.978.446.48E-058.241.02
TOM1 (A)1.99E-049.79E-050.492.912.27E-052.891.01
RRN3 (A)2.49E-056.00E-052.411.781.44E-051.830.97
YLR307C-A (B)1.77E-049.16E-050.522.722.21E-052.810.97
MNN4 (A)1.76E-046.86E-050.392.031.72E-052.190.93
NDD1 (A)2.42E-067.97E-0532.932.362.11E-052.680.88
HOP1 (A)1.60E-041.21E-040.763.63.25E-054.130.87
REV7 (A)1.58E-048.95E-050.572.662.52E-053.210.83
RLI1 (C)1.90E-041.36E-040.724.023.83E-054.870.83
HAC1 (A)5.12E-059.18E-051.792.722.65E-053.370.81
CDC26 (A)3.38E-059.59E-052.842.842.97E-053.780.75
DUN1 (A)5.06E-051.51E-042.984.485.14E-056.540.69
TSC11 (C)9.44E-051.05E-041.113.113.72E-054.730.66
SDC25 (D)8.31E-051.32E-041.593.94.72E-056.010.65
ATG13 (D)9.77E-056.60E-050.681.962.44E-053.10.63
CDC9 (A)4.08E-067.00E-0517.162.082.72E-053.460.6
SWE1 (A)9.25E-058.45E-050.912.513.44E-054.380.57
LEU3 (A)8.54E-055.60E-050.661.662.31E-052.940.56
YIR035C (B)6.75E-056.43E-050.951.912.76E-053.510.54
ACK1 (B)1.33E-041.23E-040.923.665.39E-056.860.53
SSK1 (D)1.18E-049.87E-050.842.934.50E-055.730.51
DST1 (A)1.89E-048.61E-050.462.553.92E-054.990.51
YKL044W (B)7.20E-051.08E-041.53.195.01E-056.370.5
IME2 (A)3.12E-055.22E-051.671.552.52E-053.210.48
SEC6 (C)1.33E-041.21E-040.913.595.89E-057.490.48
GDA1 (D)1.54E-041.49E-040.974.417.73E-059.830.45
FOB1 (A)5.99E-056.47E-051.081.923.48E-054.430.43
ECM16 (C)5.65E-051.16E-042.053.466.49E-058.260.42
SYF1 (A)3.31E-065.63E-0517.011.673.16E-054.020.42
SPO12 (A)7.20E-055.68E-050.791.693.59E-054.570.37
BFR2 (C)9.86E-046.78E-050.072.014.48E-055.70.35
MSC1 (A)4.22E-055.68E-051.351.683.87E-054.920.34
SPE4 (A)2.98E-064.99E-0516.741.483.48E-054.430.33
DBR1 (A)5.76E-051.10E-041.913.267.79E-059.910.33
SIR4 (A)1.76E-041.22E-040.693.628.66E-0511.020.33
DIP5 (A)1.06E-049.95E-050.942.957.31E-059.30.32
TEA1 (A)1.36E-043.93E-050.291.172.91E-053.70.32
STU2 (A)1.60E-045.34E-050.331.583.98E-055.060.31
ELG1 (A)5.10E-056.85E-051.342.035.12E-056.510.31
LHS1 (A)2.34E-046.27E-050.271.864.98E-056.340.29
RAD55 (A)8.48E-058.52E-0512.537.84E-059.970.25
NGR1 (A)1.37E-042.29E-060.020.072.29E-060.290.24
RAD1 (A)2.51E-047.43E-050.32.27.17E-059.120.24
NMD2 (D)1.47E-041.03E-050.070.311.03E-051.310.24
PPH21 (A)2.01E-052.30E-051.140.682.30E-052.930.23
PTC3 (A)9.81E-054.76E-050.491.414.89E-056.220.23
UTP22 (C)9.52E-054.69E-050.491.395.00E-056.370.22
RFC4 (A)1.78E-046.64E-050.371.977.33E-059.330.21
HIR3 (A)1.72E-044.45E-050.261.326.96E-058.850.15
MRC1 (A)4.42E-052.89E-050.650.867.37E-059.380.09
YDR066C (B)6.18E-053.13E-050.510.939.13E-0511.620.08
ATG11 (D)1.61E-042.22E-050.140.667.70E-059.80.07
TRX1 (A)1.22E-043.78E-050.311.121.44E-0418.320.06
SPE1 (D)1.69E-041.18E-040.73.56.87E-0487.40.04
YAL066W (B)6.74E-051.33E-041.973.957.83E-0499.620.04
MCM21 (A)8.63E-054.21E-060.040.124.03E-03512.720
  • Column 1 identifies the GOI isolated in the mutagenesis screen by transposon insertion and selected for construction of a precise heterozygous deletion (goiΔ/GOI) in both rad9-deficient and wild-type backgrounds. The basis for inclusion of this gene in this subset is denoted by the letter in parentheses (A, B, C, and D) after the gene name. A, gene ontology statistically significantly overrepresented group; B, hypothetical open reading frame; C, essential; D, high chromosome V instability rate (μ). Column 2 shows μ (events/cell/generation) as measured by conversion to canavanine resistance of the insertionally mutagenized strains (ISM) in a rad9Δ/rad9Δ background. Column 3 shows the μ of the precise heterozygous deletion in the rad9-deficient background. Column 4 shows the ratio between the two to determine the reproducibility of the instability phenotype. Column 5 shows the ratio (A) of the μ of the heterozygous gene deletion in the rad9Δ/rad9Δ background to the μ of the rad9Δ/rad9Δ parental strain. Column 6 shows the μ of the heterozygous deletion in a wild-type background. Column 7 shows the ratio (B) of the μ of the heterozygous gene deletion in the wild-type background to the wild-type parental strain. Column 8, the calculated A/B ratio, shows the fold change between the increases in μ due to the heterozygous gene deletion in the rad9Δ/rad9Δ vs. wild-type backgrounds. Column 8 was then used to categorize these chromosome V instability rates on the basis of whether the fold change due to the heterozygous deletion was larger or smaller in the rad9Δ/rad9Δ or wild-type backgrounds. The first group (A > B) represents 8 strains where the heterozygous deletion of the GOI appears to have a larger effect in a rad9Δ/rad9Δ background than in a wild-type background. The second group (A ≈ B) represents 12 strains where heterozygous deletion of the GOI has a similar effect in both backgrounds. The third group (A < B) represents 45 strains where the heterozygous gene deletion has a larger fold effect on chromosome V instability in the wild-type background compared to the rad9Δ/rad9Δ background.