TABLE 8

Conversion frequencies in MSH4 and msh4 backgrounds for markers making WRMs or PRMs

MATHYGKANHISNATTRPaLEUARGURAWRMb
A. YFS707: Petes, MSH4
Normal 4:4297929752829238926823003298527683009
    8:0004011043120
    6:25862204156344344823027777
    5:30004520003500
Aberrant 4:40004000010
    Σc5862208648355345226928797
% conversion1.92.06.821.311.71.11.78.90.926.2
N = 3037 four-spore viable tetrads; total dissected = 4000
B. YFS713: Petes, msh4
Normal 4:4156815661488130414021582155814851588
    8:00010400205
    6:22830107451901438938415
    5:30002260001600
Aberrant 4:400021000000
    Σc283010829219414381118420
% conversion1.81.96.818.312.20.92.47.00.526.3
    Pd0.70.70.90.020.60.40.10.020.1
N = 1596 four-spore viable tetrads; total dissected = 2400
C. YFS621; Rine, MSH4
Normal 4:42803274427052647282524992861
    8:001030004
    6:231131602265116415454
    5:3001110021300
Aberrant 4:40000030
    Σe311321712295137715459e
% conversion1.14.66.18.01.813.20.616.0
N = 2876 (2834 for MAT) four-spore viable tetrads; total dissected = 4020
D. YFS636: Rine, msh4
Normal 4:41397133512811251138711691416
    8:0030711011
    6:2278630166361018323
    5:3001130015300
Aberrant 4:40030030
    Σc2789143173372558334
% conversion1.96.310.312.12.618.10.623.5
N = 1576 four-spore viable tetrads; total dissected = 3000
  • a The TRP insertion, included here for its contribution to these conversion data, proved to be too close, in centimorgans, to the LEU-ARG4-URA interval to give useful interference data.

  • b Sum for the row minus conversions for the markers (at HIS and ARG) making PRMs.

  • c Sum of conversions, including aberrant 4:4's.

  • d The probability that the differences in conversion frequencies between the MSH4 and msh4 crosses are attributable to chance alone.

  • e Raised by one to normalize for tetrads lost in the MAT column.