TABLE 3

Effects of daf-2, daf-18, and daf-16 on life span

GenotypeMean (days) ±SEMedian (days)Maximum (days)NaPbPc
Trial 1
+15.5 ± 0.5152255 (84)
daf-18(nr2037)8.2 ± 0.371363 (102)<0.0001
daf-16(mgDf50)10.6 ± 0.391768 (80)<0.0001
daf-2(e1370)29.9 ± 2.4354221 (77)<0.0001
daf-2(e1370); daf-18(0)9.9 ± 0.3101377 (94)<0.0001<0.0001
daf-2(m646); daf-18(0)13.2 ± 0.4132179 (102)<0.0083<0.0001
daf-16(0); daf-2(m646)9.8 ± 0.391684 (84)<0.00010.1423
Trial 2
+16.2 ± 0.5182162 (75)
daf-18(nr2037)9.2 ± 0.1101087 (100)<0.0001
daf-16(mgDf50)11.2 ± 0.11111100 (100)<0.0001
daf-2(m65); daf-18(0)d 8.2 ± 0.571472 (75)<0.00010.6757
daf-16(0); daf-2(m65)9.7 ± 0.4111495 (98)<0.00010.6486
Trial 3
+16.3 ± 0.6162660 (60)
daf-18(nr2037)10.4 ± 0.3121489 (100)<0.0001
daf-16(mgDf50)13.4 ± 0.2141493 (100)<0.0001
daf-2(m65); daf-18(0)28.9 ± 0.5293795 (100)<0.0001<0.0001
daf-16(0); daf-2(m65)14.2 ± 0.2141697 (100)<0.0001<0.0002
Trial 4
+13.4 ± 0.3131772 (75)
daf-18(nr2037)8.4 ± 0.2101177 (100)<0.0001
daf-2(m65); daf-18(0)17.6 ± 0.6172690 (100)<0.0001<0.0001
daf-2(m631); daf-18(0)14.9 ± 0.3171990 (100)<0.0001<0.0001
daf-2(m646); daf-18(0)13.6 ± 0.3141496 (100)0.0284<0.0001
Trial 5
+19.4 ± 0.4182899 (100)
daf-16(mgDf50)12.9 ± 0.11216100 (100)<0.0001
daf-16(0); daf-2(m65)12.3 ± 0.3122090 (100)<0.00010.3922
daf-16(0); daf-2(m646)12.2 ± 0.1121499 (100)<0.0001<0.0001
daf-16(0); daf-2(tm1236)12.4 ± 0.1121696 (100)<0.00010.0041
Trial 6
+16.2 ± 0.2162098 (100)
daf-16(mgDf50)12.2 ± 0.1121699 (100)<0.0001
daf-16(0); daf-2(m65)11.1 ± 0.3121896 (100)<0.00010.0552
daf-16(0); daf-2(m646)12.2 ± 0.2122099 (100)<0.00010.5758
daf-16(0); daf-2(tm1236)12.6 ± 0.1121696 (100)<0.00010.0138
  • FUdR was used for trials 2–4 to prevent death due to internal hatching of eggs, which affected some of the double-mutant strains. Maximum life span is the last day on which live worms were observed.

  • a Deaths scored (initial sample size).

  • b Probability that life span is identical to that of wild type with same temperature history (log rank test).

  • c Probability that life span is identical to that of single Daf-d mutant (log rank test).

  • d In trial 2, daf-2(m65); daf-18(0) is not longer lived than daf-18(0), in contrast to trials 3 and 4. While the reason for this discrepancy is unknown, we believe that the latter results are correct, given that daf-2(m631) and daf-2(m646) also increase daf-18(0) life span and that the severity ranking of the effects of these three daf-2 alleles is the same for both life span and larval arrest.