Mutagenesis and mitotic recombination in strains with PCNA interaction-defective alleles of MLH1 and MSH6

CAN1 forward mutationaRecombination rate × 108
Relevant genotypeRate × 108(C.I.)Relative to WTHR(C.I.)HER(C.I.)HER:HR rateb
WT11.0(7.2–13.6)1.0213(198–232)16.6(14.4–19.3)0.078 (0.062–0.98)1.0
mlh1Δ1110(768–1480)101222(169–306)102(56.2–159)0.46 (0.18–0.94)5.9
mlh1-QLF508(444–818)57.7192(144–208)54.4(43.9–64.5)0.28 (0.21–0.45)3.6
Plasmid-encoded allele in msh6Δ strainsc
MSH617.3(13.4–18.6)1.091.3(78.8–99.3)7.67(6.31–9.48)0.084 (0.064–0.12)1.0
None973(765–1160)56.2108(92.8–131)75.9(66.3–96.1)0.70 (0.51–1.0)8.3
msh6Δ2-251402(272–466)23.292.2(73.5–106)13.5(11.9–15.7)0.15 (0.11–0.21)1.8
msh6Δ51-25137.2(21.5–40.0)2.15103(68.0–135)9.50(8.00–14.2)0.092 (0.059–0.21)1.1
msh6-FFAA,Δ51-251236(151–279)13.6135(96.3–151)11.5(9.30–15.6)0.085 (0.062–0.16)1.0
  • C.I. = 95% confidence interval; WT, wild type.

  • a The Can-R rates were calculated by pooling data from the HR and HER strains of a given genotype.

  • b Ninety-nine percent confidence limits of the HER:HR ratios are shown in parentheses and were calculated as described in materials and methods. The underlined numbers are the HER:HR rates normalized to WT.

  • c Alleles were encoded by the following plasmids: MSH6 by pRDK3572, msh6Δ2-251 by pRDK4650, msh6Δ51-251 by pRDK4715, and msh6-FFAA,Δ51-251 by pRDK4758 (Shell et al. 2007). HR and HER rates were measured in the SJR1856 and SJR1850 strain backgrounds, respectively.