TABLE 4

Meiotic segregation of the his4-AAG marker

Sporulation temperatureStrainRelevant genotypeNo. tetradsAb tetrads (%)PMS tetrads (%)PMS/Ab events (%)
30°PD83WT335191.26.1
No. 5msh2Δ79201472
JSY175pol30-5274144.130d
18°PD83aWT482581018
No. 5bmsh2Δ111645590
JSY346pol30-201336341652e
JSY354pol30-201 msh2Δ184352779e
DB101/HMY95amlh1Δ465574989
SJR2577 × SJR2578mlh1-QLF226513878f
SJR2705 × SJR2706cWT165632130
SJR2705 × SJR2707cmsh6-FFAA,Δ51-251305603862g
  • The columns of % events are as follows: % Ab tetrads, percentage of total tetrads with aberrant (non-4:4) segregation; % PMS tetrads, percentage of total tetrads with one or more PMS events, excluding tetrads with one gene conversion event and one PMS event (7:1, 1:7); % PMS/Ab events, percentage of aberrant events that are PMS events, calculated as described by Stone and Petes (2006). Two-tailed Fisher's exact tests were used to determine P-values and P-values were considered significant when <0.05. WT, wild type.

  • a Data from Welz-Voegele et al. (2002).

  • b Data from Kirkpatrick and Petes (1997).

  • c Alleles were encoded by the following plasmids: MSH6 by pRDK3572 and msh6-FFAA,Δ51-251 by pRDK4758 (Shell et al. 2007). To ensure that the plasmid was present during meiosis, only those tetrads that had at least one Leu+ spore were scored.

  • d Significant increase from PD83 (P = 0.04) and significant decrease from JSY200/No. 5 (P = 0.05) in the number of PMS vs. the number of non-PMS aberrant events.

  • e Significant increase from PD83 (P < 0.001) and significant decrease from JSY200/No. 5 (P < 0.001) in the number of PMS vs. the number of non-PMS aberrant events.

  • f Significant increase from PD83 (P < 0.001) and significant decrease from DBY101/HMY95 (P = 0.003) in the number of PMS vs. the number of non-PMS aberrant events.

  • g Significant increase from the WT SJR2705 × SJR2706 control (P < 0.001).