TABLE 2

CAN1 forward mutation rates in pol30 haploid strains

BackgroundRelevant genotypeRate × 108(C.I.)Relative to WT
SJR328aWT8.8(7.9–9.3)1.0
msh3Δ msh6Δ481(422–513)54.6
pol30-521080(437–1400)123
pol30-104258(196–298)29.3
pol30-20168.8(62–121)7.8
pol30-20450.6(39.2–61.7)5.7
pol30-201 msh3Δ msh6Δ499(295–624)56.6
pol30-201, 204728(626–801)82.6
AS13bWT14.1(11.6–16.2)1.0
msh2Δ252(234–282)17.9
pol30-52908(707–1390)64.4
msh2Δ pol30-524120(2400–5260)292
pol30-201173(149–195)12.3
pol30-201 msh2Δ342(325–394)24.3
AS4bWT11.7(11.0–16.5)1.0
msh2Δ193(179–209)16.5
pol30-521290(857–1830)110
  • WT, wild type; C.I. = 95% confidence interval.

  • a Rates determined by combining data from the cβ2-100% and cβ2-4ns strains (see Table 1 for strain names).

  • b Strain names for the AS13 derivatives were PD73 (WT), RKY1452 (msh2Δ), JSY162 (pol30-52), JSY222 (msh2Δ pol30-52), SJR2203 (pol30-201), and JSY332 (pol30-52 msh2Δ); names for the AS4 derivatives were JSY208 (WT), JSY203 (msh2Δ), and JSY209 (pol30-52).