TABLE 1

Rates and classes of germinal revertants isolated from a1-m5216 in RAD51+ plants

CrossaNo. perfect excisionsNo. imperfect excisionsTotal no. revertantsPopulation sizeRate of germinal revertants
Cross 17b3c10347,3002.88 × 10−5
Cross 23d03110,0002.72 × 10−5
Cross 32e1f3157,0001.91 × 10−5
Totalg12h416614,3002.60 × 10−5
  • a Refer to materials and methods.

  • b Type 5 in Figure 3A (plant identification nos. 03B-17-3, 03B-38-6, 03B-47-4, 03B-49-6, 03B-93-5, 03B-66-7, 02B-1132-L).

  • c All three types are shown in Figure 3A [plant identification nos. 03B-16-2 (type 1), 03B-45-1 (type 2), 03B-68-3 (type 3)].

  • d Type 5 in Figure 3A (plant identification nos. 03B-56-1, 03B-60-4, 03B-61-4).

  • e Type 5 in Figure 3A (plant identification nos. 02B-1185 and 02B-1188).

  • f Type 4 in Figure 3A (plant identification nos. 02B-1181).

  • g No significant differences were detected among these three populations based on the chi-square homogeneity test. Therefore, data from the three crosses were pooled.

  • h The high proportion of perfect excision events is probably due to selection, in that only colored kernels from crosses 1–3 were analyzed.