TABLE 4

Relative levels of Psod-3:gfp expression in daf-2 mutants subjected to germline or whole-gonad ablation

StrainGenotypePsod-3∷gfp expression relative to intactnPPMean life span (days)nPP
CF1580daf-2(e1370)Intact1 ± 0.0422343.2 ± 0.93a245
Germ cell (−)1.52 ± 0.06317<0.00164.5 ± 3.5a59<0.001
Whole gonad (−)1.98 ± 0.08317<0.001<0.00169.5 ± 6.0a31<0.0010.33
CF2533daf-2(e1368,RNAi)Intact1 ± 0.191451.0 ± 1.9b68
Germ cell (−)1.17 ± 0.13110.45687.90 ± 4.437<0.001
Whole gonad (−)1.76 ± 0.18150.0070.014124.1 ± 5.9b39<0.001<0.001
CF1553daf-2(RNAi)Intact1 ± 0.191836.5 ± 1.7113
Germ cell (−)5.15 ± 0.6016<0.00172.8 ± 2.573<0.001
Whole gonad (−)3.09 ± 0.60210.0030.02058.2 ± 2.280<0.001<0.001
CF2533daf-2(e1368)Intact1 ± 0.0343734.3 ± 1.0a110
Germ cell (−)3.74 ± 0.1935<0.00171.0 ± 2.3a45<0.001
Whole gonad (−)3.48 ± 0.1940<0.0010.3741.1 ± 1.5a45<0.001<0.001
CF1588daf-16(mu86); daf-2(e1370)Intact1 ± 0.04425
Germ cell (−)0.74 ± 0.02928<0.001
Whole gonad (−)0.93 ± 0.042250.26<0.001
CF2683daf-16(mu86); daf-2(e1368)Intact1 ± 0.06719
Germ cell (−)0.85 ± 0.064150.11
Whole gonad (−)0.88 ± 0.044110.140.69
  • Psod-3∷gfp expression values represent mean fluorescence intensity relative to intact controls. P represents the P-value (Student's t-test for Psod-3∷gfp expression and Logrank Mantel/Cox for life span data) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

  • a Life span data previously published in Hsin and Kenyon (1999), and included here for comparison.

  • b Life span data previously published in Arantes-Oliveira et al. (2003) and included here for comparison.