TABLE 2

daf-16 dependence of gene expression in animals lacking germ cells

ConstructConstruct no.StrainTrialdaf-16 genotypeIntactnPGerm cell (−)nPWhole gonad (−)nPP
sod-3∷gfpmuIs84CF18741daf-16(mu86)1 ± 0.044290.79 ± 0.044260.0010.87 ± 0.042240.0340.17
gpd-2∷gfpsEx11128BC111281daf-16(RNAi)1 ± 0.087200.84 ± 0.13190.3010.66 ± 0.11180.0190.29
BC11128daf-16(+)0.9 ± 0.077190.421.5 ± 0.16170.005
BC111282daf-16(RNAi)1 ± 0.087250.55 ± 0.1116<0.0010.24 ± 0.06227<0.0010.14
BC11128daf-16(+)0.63 ± 0.068250.0021.8 ± 0.1221<0.001
dod-8∷gfpsIs10314CF26761daf-16(mu86)1 ± 0.077241.8 ± 0.1417<0.0012.3 ± 0.1527<0.0010.016
CF2630daf-16(+)1.3 ± 0.077300.006
CF26762daf-16(mu86)1 ± 0.19212.7 ± 0.44180.0024.1 ± 0.399<0.0010.023
CF2630daf-16(+)1.1 ± 0.15240.678.6 ± 0.6925<0.001
dod-8∷rfpmuEx405CF29221daf-16(mu86)1 ± 0.14172.3 ± 0.2924<0.0011.6 ± 0.19280.0110.07
CF2760daf-16(+)a0.84 ± 0.13200.424.0 ± 0.3318<0.0012.4 ± 0.3317<0.001
CF29222daf-16(mu86)1 ± 0.12122.0 ± 0.30130.0092.6 ± 0.42170.0010.19
CF2760daf-16(+)b1.7 ± 0.23130.0163.6 ± 0.3216<0.0013.7 ± 0.3812<0.001
nnt-1∷gfpsEx10466BC104661daf-16(RNAi)1 ± 0.21191.5 ± 0.23280.1252.2 ± 0.2640<0.0010.03
BC10466daf-16(+)1.2 ± 0.24160.482.8 ± 0.4117<0.001
CF29232daf-16(mu86)1 ± 0.17352.6 ± 0.3239<0.0012.7 ± 0.4033<0.0010.88
BC10466daf-16(+)c0.56 ± 0.15310.055.4 ± 0.78723<0.0016.2 ± 0.9625<0.001
  • Values represent mean fluorescence intensity relative to intact daf-16(−) controls. Fluorescence was measured on day 2 of adulthood. P represents the P-value (Student's t-test) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

  • a Same data labeled trial 4 for dod-8∷rfp in Table 1.

  • b Same data labeled trial 5 for dod-8∷rfp in Table 1.

  • c Same data labeled trial 4 for nnt-1∷gfp in Table 1.