TABLE 1

The somatic gonad is required for DAF-16 to activate some of its target genes in animals that lack germ cells

ConstructConstruct no.StrainTrialIntactnGerm cell (−)nPWhole gonad (−)nPP
sod-3∷gfpamuIs84CF155311 ± 0.048348.0 ± 1.1726<0.0011.6 ± 0.1232<0.001<0.001
gpd-2∷gfpsEx11128BC1112811 ± 0.12192.0 ± 0.18517<0.0010.53 ± 0.073160.002<0.001
21 ± 0.076280.91 ± 0.1226<0.0010.60 ± 0.04232<0.0010.031
31 ± 0.12191.47 ± 0.18130.040.76 ± 0.14180.210.004
dod-8∷gfpsIs10314CF263011 ± 0.007282.17 ± 0.02638<0.0012.3 ± 0.01732<0.0010.60
21 ± 0.113615.9 ± 1.535<0.0019.9 ± 0.4946<0.001<0.001
31 ± 0.10303.3 ± 0.3527<0.0012.5 ± 0.1533<0.0010.033
41 ± 0.086313.1 ± 0.2234<0.0012.2 ± 0.09135<0.001<0.001
dod-8∷rfpmuEx405CF276011 ± 0.13272.8 ± 0.1926<0.0011.9 ± 0.1438<0.001<0.001
21 ± 0.12344.2 ± 0.4037<0.0014.2 ± 0.3543<0.0010.92
31 ± 0.17284.8 ± 0.3435<0.0014.0 ± 0.4421<0.0010.14
4b1 ± 0.16204.8 ± 0.4018<0.0012.9 ± 0.4017<0.0010.002
5c1 ± 0.13132.2 ± 0.1916<0.0012.2 ± 0.2312<0.0010.93
nnt-1∷gfpsEx10466BC1046611 ± 0.25236.0 ± 0.9223<0.0015.1 ± 0.92230.0020.57
21 ± 0.23343.5 ± 0.4329<0.0014.2 ± 0.4735<0.0010.25
31 ± 0.14309.1 ± 1.032<0.0016.4 ± 0.8029<0.0010.04
4d1 ± 0.27319.6 ± 1.423<0.00111 ± 1.725<0.0010.51
  • Values represent mean fluorescence intensity relative to intact controls. Fluorescence was measured on day 2 of adulthood. P represents the P-value (Student's t-test) compared to intact control, and P′ represents the P-value compared to animals lacking germ cells (Z2 and Z3 ablated).

  • a Changes in Psod-3∷gfp expression were observed twice by eye and quantification was performed on the third trial.

  • b Same data labeled trial 1 of dod-8∷rfp in Table 2.

  • c Same data labeled trial 2 of dod-8∷rfp in Table 2.

  • d Same data labeled trial 2 of nnt-1∷gfp in Table 2.