TABLE 1

Karyotyping markers included in the eight-probe mix

DNA sequencesFluorescent moleculesIndividual cosmid use final concentration (ng/μl)19-cosmid mix use final concentration (ng/μl)
Knob 180 bpCoumarin or Cascade Blue12.515.1
4-12-1FITC or Alexa Fluor 48812.515.1
NOR-173FITC or Alexa Fluor 4880.06250.08
Cent CFITC or Alexa Fluor 4880.50.6
2-3-3FITC or Alexa Fluor 4883.1253.8
Cent 4Cy512.515.1
1-26-2Cy56.257.5
2-3-3Cy56.257.5