TABLE 1

Characteristics of the deduced mature proteins of the 1Dx5*t and 1Dx51*t subunits compared to previously reported HMW-GSs from Ae. tauschii and common wheat

No. of amino acid residues and repeat unit variations
RD
Tripeptide variantsHexapeptide variantsNanopeptide variants
123123456123456789No. of Cys residuesMW (Da)
SubunitsNDGQQV/TaPGQGQQV/TaGYYPTSLQQV/TaV/T%bCDMNDRDCDTotal
Dx5*t891112/20201001301646/70201200122314/1967.41420.91301485,782
Dx5.1*t891213/21231311421652/72101200153316/1975.82421.03301487,663
Dx5891112/22201311431850/71201201153415/1972.22421.08311587,189
Dx2881213/20211101421549/72101200153315/1970.32420.97301487,023
Dx2.1t892113/21201101321647/71201200152315/1968.89420.97301488,914
Dx2.2t891213/23201501512054/81101200184318/2170.59420.983014103,048
Dx2t891112/2120901221645/71101200152315/1966.67420.92301488,700
Dx3t891112/2120911321645/71101210152315/1966.67420.96301488,829
Dx5.2t891112/21211101331145/71111200142415/1966.67420.93311586,779
Dy121041863136530/501102180100215/1965.22421.21511768,711
Dy101041563124525/481102170100215/1959.70421.15511767,495
  • M, average number of variants per unit, including hexapeptides and nonapeptides. ND, N-terminal domain. RD, repetitive domain. CD, C-terminal domain. MW, molecular weights calculated from the derived amino acid sequences.

  • a Variant motif number/total motif number.

  • b Percentage of variant motifs relative to total motifs.