TABLE 2

Dominant-negative spt3 mutations

spt3 alleleaPredicted amino acid changebStrength of phenotypec
spt3-143W143RStrong
spt3-178L178RStrong
spt3-179K179EStrong
spt3-182D182G (2×)Strong
spt3-188M188V (2×)Medium
spt3-189M188TStrong
spt3-190M188IStrong
spt3-193Y193CStrong
spt3-194Y193FStrong
spt3-195Y193HStrong
spt3-196W196RStrong
spt3-197W196CStrong
spt3-201Q201PStrong
spt3-204A202VStrong
  • a The allele number corresponds to the codon altered except in cases of multiple changes in the same codon or previous use of the allele number.

  • b In cases of multiple isolates, the number of times isolated is shown in parentheses.

  • c Strength reflects the degree of growth on SC-His medium, scored at 30° over 3 days.