TABLE 3

Phenotypes of let-60(ga89gf)

% hermaphrodite gonad arms with indicated phenotype
Oocyte phenotypes
GenotypeDisorganizedSmall oocytes, multiple rowsEmoEctopic dpMPK-1Mitotic zone sizecn
20°a
Wild type000021 ± 1.820
let-60(ga89gf)100010021 ± 1.630
25° shift-upb
Wild type000021 ± 1.620
let-60(ga89gf)40601710020 ± 1.530
  • let-60(ga89gf) is described in Eisenmann and Kim (1997). let-60(n1046gf) (Beitel et al. 1990; Han et al. 1990) hermaphrodites were also examined: First, mutant hermaphrodites are fertile at 15°, 20°, and 25°. At 20°, 58% of gonad arms have a mild disorganized oocyte phenotype (n = 31). At all temperatures, mutants lay small embryos. Time-lapse video analysis at 20° shows that the small embryos primarily arise from premature closure of the proximal gonad/distal spermathecal valve, resulting in the maturing oocyte being chopped during ovulation into the spermatheca. Second, the levels and spatial pattern of dpMPK-1 are similar in let-60(n1046gf) and wild type (see supplemental Figure 5 at http://www.genetics.org/supplemental/).

  • a Animals were picked at mid-L4 and 24 hr later gonads were obtained by dissection.

  • b Animals were picked at mid-L4, grown for an additional 20 hr at 20°, and shifted to 25° for 4 hr; then gonads were obtained by dissection.

  • c Length of the mitotic zone, in cell diameters from the distal tip, on the basis of DAPI nuclear morphology.