ceh-39 mutations enhance XX-specific phenotypes caused by other XSE mutations
| Genotypea | Survivor phenotype | Hermaphrodite viability (%)b | nc |
|---|---|---|---|
| ceh-39(y414) | Wild type | 101 | 1008 |
| ceh-39(gk296) | Wild type | 102 | 1021 |
| ceh-39(RNAi) | Wild type | 100 | 807 |
| ceh-21(RNAi) | Wild type | 100 | 1147 |
| fox-1(y303) | Wild type | 99 | 1054 |
| sex-2(y324) | Wild type to mild Dpy | 99 | 1032 |
| sex-1(y263) | Dpy, Egl Tra | 70 | 884 |
| sex-1(RNAi) | Very Dpy, Egl, Tra | 17 | 1090 |
| sex-1(y263, RNAi) | Very Dpy, Egl, Tra | 17 | 1304 |
| sex-2(y324) sex-1(y263)d | Very Dpy | 4 | 238 |
| fox-1(y303) sex-1(y263)e | Very Dpy, Egl, Tra | 4 | 1176 |
| fox-1(y303) sex-1(RNAi) | Very Dpy, Egl, Tra | 9 | 749 |
| ceh-39(y414) sex-1(y263)f | Very Dpy, Egl, Tra | 7 | 982 |
| ceh-39(y414) sex-1(y263)e | Very Dpy, Egl, Tra | 5 | 941 |
| ceh-39(RNAi) sex-1(y263) | Very Dpy, Egl | 10 | 832 |
| ceh-39(y414) sex-1(RNAi) | Dead | 0 | 935 |
| ceh-39(gk296) sex-1(RNAi) | Dead | 0 | 1637 |
| ceh-21(RNAi) sex-1(y263) | Dpy, Egl | 75 | 1043 |
| ceh-39(y414) fox-1(RNAi) | Mild Dpy | 98 | 1486 |
| ceh-39(gk296) fox-1(RNAi) | Mild Dpy | 101 | 1256 |
| ceh-39(RNAi) fox-1(y303) | Mild Dpy | 100 | 729 |
| ceh-39(RNAi) sex-2(y324) | Dpy, Egl | 99 ± 2g | 1368 |
| fox-1(y303) sex-2(y324) | Dpy, Egl | 98 ± 1g | 1010 |
| ceh-39(RNAi) fox-1(y303) sex-2(y324) | Dpy, Egl | 92 ± 1g | 1180 |
↵a RNAi was applied as explained in Table 1, footnote a.
↵b Hermaphrodite viability was calculated by the following formula: (no. of adult hermaphrodites)/(total no. of embryos) × 100.
↵c n is the total number of embryos from six independent sets of progeny counts.
↵d Data are from C. Y. Loh and B. J. Meyer (personal communication). Of 951 progeny from sex-2(y324) sex-1(y263)/szT1 animals, only 9 (of an expected 238) lacked szT1, implying that they were sex-2(y324) sex-1(y263) and only 4% were viable. sex-2(y324) sex-1(y263) animals were severely Dpy and produced no or few progeny.
↵e Percentage viability of fox-1(y303) sex-1(y263) XX progeny from the strain fox-1(y303) sex-1(y263)/szT1 and percentage viability of ceh-39(y414) sex-1(y263) XX progeny from the strain ceh-39(y414) sex-1(y263)/szT1 were calculated by the following formula: (no. of Dpy hermaphrodites)/0.25(n − no. of males) × 100. The szT1 balancer acts as a mild dominant him mutation, making it necessary to calculate the expected number of XX adults by subtracting the number of XO male progeny from the total number of embryos.
↵f This strain is maintained under yEx483[dpy-30∷sdc-2(+); myo-2∷gfp(+)], an extrachromosomal array that rescues XSE mutants because it overexpresses sdc-2. To score viability, progeny from gfp(−) hermaphrodites that had lost yEx483 were counted.
↵g Viability was calculated separately for six independent sets of progeny counts. Average viability and error are reported. Error is expressed as the standard error of the mean.