TABLE 4

Allele-specific interactions between old and new spt3 and spt15 mutations

Relevant genotypeGalahis4-917δblys2-173R2c
1. Wild type+d+
2. spt3-193−/++
3. spt3-401+++
4. spt3Δ+
5. spt15-21+
6. spt15-180++
7. spt15-239++
8. spt3-193 spt15-180++
9. spt3-193 spt15-239++
10. spt3-193 spt3-401++/−+
11. spt3-193 spt15-21+
12. spt3-401 spt15-180+
13. spt3-401 spt15-239+
14. spt3-401 spt15-21++
15. spt3Δ spt15-180+
16. spt3Δ spt15-239+
17. spt3Δ spt15-21+
  • a The Gal phenotype was tested on YPgal plates containing antimycin A.

  • b In strains with an otherwise wild-type background, his4-917δ confers a His phenotype. Suppression was tested by spotting cells on SC-His medium.

  • c In strains with an otherwise wild-type genotype, lys2-173R2 confers a Lys+ phenotype. Suppression was tested by spotting cells on SC-Lys medium.

  • d Symbols indicate growth as follows: +, full growth of the spotted cells in one day; −, no growth or extremely poor growth; +/− and −/+, intermediate levels of growth.