TABLE 1

Plasmids used in this study

PlasmidPromoterMarkerCopy numberSource
pCUP1-GFPCUP1URA3CEN, lowPark et al. (2006)
pCUP1-NMGFPCUP1URA3CEN, lowPark et al. (2006)
pRS313CUP1-GFPCUP1HIS3CEN, lowDerkatch et al. (2001)
pRS313CUP1-NMGFPCUP1HIS3CEN, lowDerkatch et al. (2001)
p426GPDSSE1GPDURA32μ highMorano lab
p423STI1STI1HIS32μ highSong et al. (2005)
pGAL-NMGFPGALHIS3CEN, lowPark et al. (2006)
p2UGHSP82GPDURA32μ, highLindquist lab
pKAT6GPDHIS32μ, highLindquist lab
sse1Δ∷LEU2LEU2Replacement, singleMorano lab
p414TEF SSE1TEFTRP1CEN, lowMorano lab
p414TEF SSE1K69QTEFTRP1CEN, lowMorano lab
p414TEF SSE1G233DTEFTRP1CEN, lowMorano lab
p414TEF SSE1PBDTEFTRP1CEN, lowMorano lab
pRS305-HSF1HSF1LEU2Integrating, singleThis study
pRS305CTAHSF1HSF1LEU2Integrating, singleThis study